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A great deal of research has been carried out on this important class of compounds in the last ten years. To ensure that scientists are kept up to date, the editors of the First Edition of The Lipid Handbook have completely reviewed and extensively revised their highly successful original work. The Lipid Handbook: Second Edition is an indispensable resource for anyone working with oils, fats, and related substances.
Fundamentals and Techniques
Comprehensive Biochemisty, Volume 16: Hydrolytic Reactions; Cobamide and Biotin Coenzymes treats both the chemistry and function of the coenzymes. This book discusses the thermodynamic and kinetic aspects of enzyme catalysis, hydrolytic enzymes displaying "active center characteristics, chelation and stereochemical considerations in enzyme catalysis, and biological oxidation mechanisms. The recommendations of the Enzyme Commission of the International Union of Biochemistry and classified list of enzymes are also covered. This publication likewise elaborates the spectral characteristics of the cobamide coenzymes and mode of binding of biotin. Other topics include the classification of carboxyl esterases, structure and reactivity of phosphorylated metabolites, and mechanism of action of chymotrypsin. This volume is a good reference for biochemists and specialists concerned with enzymes.
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology
Within recent years, affinity chromatography has become established as one of the most potent separatory techniques available to the biochemist. The technique has been exploited in almost every area of biochemistry and its applications are rapidly infiltrating cellular biology, immunology, medicine and technology. Because of this explosive development, this book is not intended as a comprehensive account of all the available systems but rather as a guide to current trends. However, whilst the detailed methodology of these systems vary, they are based on common principles.The aim of this book is to delineate these common principles and show how they are applied in practice.
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Ether Lipids: Biochemical and Biomedical Aspects is a 21-chapter text that explores the procedures for the analysis, synthesis, and biomedical application of ether lipids. This book surveys first the chromatographic analysis of ether lipids and their characterization by mass spectrometry. The following chapter describes the chemical synthesis of lipids containing alkyl or 1-alkenyl chains. Three chapters are devoted to the biosynthesis of ether lipids, and four others cover unusual ether lipids. Significant chapters focus on biologically active ether lipids, including the naturally occurring platelet-activating factor and synthetic alkyl analogs of lysophosphatidylcholines, which exhibit cancerostatic properties. This text further discusses the application of these and related substances in biomedical research as well as in clinical diagnosis and therapy. The remaining chapters highlight the use of ether lipids in studies aimed at elucidating the structure of membranes and their functions. Organic chemists, biochemists, and researchers in the biomedical field will find this book invaluable.