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The Second Edition reviews the impressive progress that has been made in the last ten years in cytochrome P450 research. Chapters emphasize structure, mechanism, biochemistry, and regulation. This edition offers expanded coverage of P450 crystal structures, site-specific mutagenesis as a probe of structure and function. New chapters explore the hormonal regulation of P450 enzymes, the cytochrome P450-catalyzed oxidation of eicosanoids, human P450 enzymes, and thiolate-ligated hemoproteins other than P450.
From his first starring role in "Just William to the huge TV successes with "The Sweeney and Minder, Dennis Waterman had an amazing theatrical career, which has also combined with an equally dramatic love life. There were affairs with Suzy Kendall and Romy Schneider, and some failed marriages, the last being with Rula Lenska. Now Waterman wants to set the record straight about his rumbustious, action-packed life. "From the Paperback edition.
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Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technol...