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This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Mitochondrial DNA: Methods and Protocols aims to be useful and informative for researchers and clinicians with an interest in mitochondrial DNA.
The most valuable gift you can give yourself or others is feeling lighthearted. Ute Devika shows that setting our emotional landscape on happy mode is an easy practice that feels good. She offers precious insights into a variety of ways to live an enchanted life while building resilience to anxiety. Coaching, HeartMath, Energy Medicine, Laughter Yoga and Meditation are empowering paths she describes with passion. Lighthearted is an enriching book offering knowledge as well as practical exercises bound to ignite the zest that helps with breaking free from heaviness. Devika connects dots between disciplines, and weaves together a multitude of ideas and stories. She alternates among two styles:...
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