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RNA-Protein Interactions : A Practical Approach
RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to th...
mRNA Processing and Metabolism
Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this ser...
Salmonella
This book presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. It is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Each chapter provides a short overview of the topic, followed by detailed explanations of techniques.
Affinity Chromatography
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet th...
Clinical Proteomics
In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.
Neural Stem Cells
Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. This is an excellent source of information and inspiration.
RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques - Offers step-by-step lab instructions, including necessary equipment and reagents
Microbial Forensics
Microbial Forensics describes the new and growing field of Microbial Forensics- the science that will help bring to justice criminals and terrorists who use biological material to cause harm. This book describes the foundation of the field of microbial forensics and will serve as a basic primer to initiate those scientists and officials that have an interest in the topic. It covers a variety of areas from forensic science, to microbiology, to epidemiology, to bioinformatics, and to legal issues.* Provides the real science beyond that displayed on TV and in the movies * Covers not only microbes but also the biology, chemistry, physics & computer science that is used for identification.* Of relevance Internationally to military, intelligence, law enforcement, agricultural, legal and environmental fields
Stem Cell Assays
The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
Neuroinformatics
Neuroinformatics presents cutting-edge techniques for the synergistic study of neuroinformatics. The book facilitates the efforts of discovering neuroscience through the sharing of data and the use of computational models. It demonstrates the use of neuroinformatic components as a mechanism for understanding complex disorders. It contains detailed explanations, advantages, and disadvantages of traditional and non-invasive imaging methods.
