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mRNA Formation and Function
mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed. - Isolation and characterization of specific RNA binding proteins - RNA metabolism and associated regulatory proteins - RNA detection and localization - A genetic approach to RNA function
A Comparative Methods Approach to the Study of Oocytes and Embryos
Over the past twenty years, biologists have made remarkable progress in understanding each step as a single fertilized egg develops into a mature organism, and the research draws on a tremendous array of experimental techniques. A Comparative Methods Approach to the Study of Oocytes and Embryos takes a unique approach to the field, providing a hands-on guide to three of the most important experimental subjects--frogs, fruitflies, and mice. By collecting reliable techniques in a single volume, the book is both an invaluable resource for new researchers and an ideal platform for comparing these seemingly disparate biological systems. The volume contains work from many leaders in the field and spans research in genetics, molecular and cellular biology, and morphology. It includes methods for oocyte and embryo culture, microinjection, in situ hybridization, immunohistochemistry, time-lapse microscopy, intracellular ion measurements, and embryonic dissections.
Biology of Fertilization V1
Biology of Fertilization, Volume 1: Model Systems and Oogenesis is the first in a three-volume series that gathers various lines of research about reproduction in general and fertilization in particular. Knowledge about cell biology, immunobiology, biochemistry, biophysics, and molecular genetics has progressed significantly beyond our understanding of some aspects of fertilization. Components of these constitute ""model systems."" The present volume includes reviews of such systems, some relatively simple model systems in lower organisms, sex-determining mechanisms, and oogenesis. The book contains 12 chapters organized into two sections. Section I includes studies on evolution, reproductive success, and immortality of the germ line; the structures and mechanisms involved in fertilization problems; and fertilization in Paramecium. Section II on oogenesis includes studies on gamete differentiation; sex-determining role of the H-Y antigen in mammals and non-mammals; the mechanism of starfish oocyte maturation; meiotic arrest in animal oocytes; and the mitotic and meiotic aspects of the mammalian germ cell life cycle.
Handbook of Cell Signaling
Handbook of Cell Signaling, Three-Volume Set, 2e, is a comprehensive work covering all aspects of intracellular signal processing, including extra/intracellular membrane receptors, signal transduction, gene expression/translation, and cellular/organotypic signal responses. The second edition is an up-to-date, expanded reference with each section edited by a recognized expert in the field. Tabular and well illustrated, the Handbook will serve as an in-depth reference for this complex and evolving field. Handbook of Cell Signaling, 2/e will appeal to a broad, cross-disciplinary audience interested in the structure, biochemistry, molecular biology and pathology of cellular effectors. - Contains over 350 chapters of comprehensive coverage on cell signaling - Includes discussion on topics from ligand/receptor interactions to organ/organism responses - Provides user-friendly, well-illustrated, reputable content by experts in the field
Regulation of Organelle and Cell Compartment Signaling
This must-have cell signaling title will appeal to researchers across molecular biology, biochemistry, cell biology and genetics. The articles are written and edited by experts in the field and emphasize signaling to and from intracellular compartments including transcriptional responses to cytoplasmic and nuclear signaling events, chromatin remodeling and stress responses, the regulation of endoplasmic reticulum function, control of cell cycle progression and apoptosis and the modulation of the activities of mitochondria and other organelles. - Articles written and edited by experts in the field - Thematic volume covering regulation of endoplasmic reticulum function, regulation of cell cycle progression, and quality control and assurance in mitochondrion events - Up-to-date research on events in membrane proteins and proteins of intracellular matrix
Cell Lineage and Embryo Patterning
International Review of Cytology presents current advances and comprehensive reviews in cell biology, both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.This volume brings together current information on the localization and roles of RNAs in cell-lineage determination and subsequent patterning in embryonic development. Guest co-editor Lawrence Etkin is one of the leading researchers in molecular genetics of Xeno...
Nuclear Trafficking
Nuclear Trafficking is a summary of the state of knowledge in nuclear trafficking, and is organized into five parts. The book begins by discussing the diffusion and signal-mediated transport through the pores. It then looks into the detailed accounts of pore structure and composition, nuclear localization signals, signal binding proteins, RNA efflux, and biochemical factors influencing nucleocytoplasmic exchange. This book will be very useful to those people new to this field of interest.
RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. - Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway - Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression - Offers step-by-step lab instructions, including necessary equipment and reagents
