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High Pressure NMR
In recent years, there has been a major expansion of high pressure research providing unique information about systems of interest to a wide range of scientific disciplines. Since nuclear magnetic resonance has been applied to a wide spec trum of problems in chemistry, physics and biochemistry, it is not surprising to find that high pressure NMR techniques have also had many applications in these fields of science. Clearly, the high information content of NMR experiments combined with high pressure provides a powerful tool in modern chem istry. It is the aim of this monograph, in the series on NMR Basic Principles and Progress, to illustrate the wide range of prob lems which can be successfu...
Intrinsically Disordered Proteins
The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Solid State NMR Spectroscopy
This book is for those familiar with solution-state NMR who are encountering solid-state NMR for the first time. It presents the current understanding and applications of solid-state NMR with a rigorous but readable approach, making it easy for someone who merely wishes to gain an overall impression of the subject without details. This dual requirement is met through careful construction of the material within each chapter. The book is divided into two parts: "Fundamentals" and "Further Applications." The section on Fundamentals contains relatively long chapters that deal with the basic theory and practice of solid-state NMR. The essential differences and extra scope of solid-state NMR over ...
Rassismuskritische politische Bildung
Wie entsteht Rassismus? Welche Zusammenhänge lassen sich zwischen Sprache und Rassismus beobachten? Sollte rassismuskritische Bildung nicht eine Querschnittsaufgabe aller Bildungsarbeit sein? So steht vor dem Hintergrund neuer bundespolitischer Programme zur Extremismusprävention und Demokratieförderung nicht nur die non-formale politische Bildung vor neuen Herausforderungen, Klärungsprozessen und Selbstverständnisdebatten. Die Autorinnen und Autoren des Bandes widmen sich der vielschichtigen Ausdifferenzierung der rassismuskritischen Bildung: Neben dem Thema Antisemitismus werden auch Kolonialismus sowie Nachhaltigkeit und Globalisierung behandelt. Auch Rassismus im Kontext von Populismus und Rechtsextremismus kommt zur Sprache. Es werden unterschiedliche Institutionen im Hinblick auf Rassismus(-kritik) betrachtet. Zudem finden sich im Band medienpädagogisch und -analytisch orientierte Beiträge. Es wird deutlich, welchen wichtigen Beitrag politische Bildung leisten kann, um Rassismus präventiv und aktiv entgegenzutreten.
Fragment Based Drug Design
There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in...
Protein Conformational Dynamics
This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to...
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Four on a Garden
Four on a Garden is a set of four one-act plays that were presented on Broadway at the Broadhurst Theatre from January 30, 1971, until March 20, 1971. The set included House of Dunkelmayer, Betty, Toreador, and The Swingers.
