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With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Synopsis: This book examines collaboration between teachers, administrators, student support specialists, community agencies, and service providers to improve outcomes for students with complex learning needs.
After a half century of transformative economic progress that moved hundreds of millions of people out of poverty, countries in developing East Asia are facing an array of challenges to their future development. Slowed productivity growth, increased fragility of the global trading system,and rapid changes in technology are all threatening export-oriented, labor-intensive manufacturing—the region’s engine of growth. Significant global challenges—such as climate change and the COVID-19 pandemic—are exacerbating economic vulnerability. These developments raise questions about whether the region’s past model of development can continue to deliver rapid growth and poverty reduction.Agai...
This text provides expert instruction on the varying surgical techniques currently employed for the regeneration of the ocular surface. Corneal Regeneration: Therapy and Surgery begins with a thorough discussion of current research based on data obtained in clinical human studies, and discusses the potential clinical implications for this promising new stage of eye surgery. Sections devoted to the stem cell, regenerative surgery and therapy of the ocular surface epithelium, corneal stroma, and corneal endothelium follow, each section comprehensively covering applied anatomy, current therapy and regenerative techniques, with a look to future directions of the field including eventual cell therapy. Corneal Regeneration: Therapy and Surgery is the first book of its kind, systematically covering the developments the medical community has achieved in corneal regeneration from all angles. Written and edited by leading experts in the field, researchers and ophthalmologists alike will find this to be a unique source of information on corneal regeneration, as well as a thoughtful reflection on potential applications of regenerative surgery in ophthalmology as a whole.
Focusing on the specific challenges of research design and exploring the opportunities of conducting research in humanitarian logistics and supply chain management, this handbook is a significant contribution to future research. Chapters include extensive descriptions of methods used, highlighting their advantages and disadvantages, and the challenges in scoping, sampling, collecting and analysing data, as well as ensuring the quality of studies. Covering a wide variety of topics including risk and resilience and the impact of humanitarian logistics on capacity building, sustainability and the local economy, it also explores the need for scalability and co-ordination in the humanitarian network. Contributors provide important insight on future directions and offer crucial guidance for researchers conducting projects within the field.
#1 New York Times Bestseller Legendary venture capitalist John Doerr reveals how the goal-setting system of Objectives and Key Results (OKRs) has helped tech giants from Intel to Google achieve explosive growth—and how it can help any organization thrive. In the fall of 1999, John Doerr met with the founders of a start-up whom he'd just given $12.5 million, the biggest investment of his career. Larry Page and Sergey Brin had amazing technology, entrepreneurial energy, and sky-high ambitions, but no real business plan. For Google to change the world (or even to survive), Page and Brin had to learn how to make tough choices on priorities while keeping their team on track. They'd have to know...
Using case law from multiple jurisdictions, Stephen Mason examines the nature and legal bearing of electronic signatures.
Aggregation-Induced Emission (AIE) is a novel photophysical phenomenon which offers a new platform for researchers to look into the light-emitting processes from luminogen aggregates, from which useful information on structure–property relationships may be collected and mechanistic insights may be gained. The discovery of the AIE effect opens a new avenue for the development of new luminogen materials in the aggregate or solid state. By enabling light emission in the practically useful solid state, AIE has the potential to expand significantly the technological applications of luminescent materials. Aggregation-Induced Emission: Fundamentals is the first book to explore the fundamental iss...
The European experience suggests that the efforts made to achieve an efficient trade-off between monetary policy and prudential supervision ultimately failed. The severity of the global crisis have pushed central banks to explore innovative tools—within or beyond their statutory constraints—capable of restoring the smooth functioning of the financial cycle, including setting macroprudential policy instruments in the regulatory toolkit. But macroprudential and monetary policies, by sharing multiple transmission channels, may interact—and conflict—with each other. Such conflicts may represent not only an economic challenge in the pursuit of price and financial stability, but also a legal uncertainty characterizing the regulatory developments of the EU macroprudential and monetary frameworks. In analyzing the “legal interaction” between the two frameworks in the EU, this book seeks to provide evidence of the inconsistencies associated with the structural separation of macroprudential and monetary frameworks, shedding light upon the legal instruments that could reconcile any potential policy inconsistency.
High-quality primary care is the foundation of the health care system. It provides continuous, person-centered, relationship-based care that considers the needs and preferences of individuals, families, and communities. Without access to high-quality primary care, minor health problems can spiral into chronic disease, chronic disease management becomes difficult and uncoordinated, visits to emergency departments increase, preventive care lags, and health care spending soars to unsustainable levels. Unequal access to primary care remains a concern, and the COVID-19 pandemic amplified pervasive economic, mental health, and social health disparities that ubiquitous, high-quality primary care mi...