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With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Covering one of the hottest topics in immunology today, this book provides a comprehensive view of all types of regulatory T cells described so far in the literature. The book will have broad appeal to both researchers and clinicians.
Book Contains 5 major parts subdivided in chapters with themes from Evolution and Creation to our Government and Financial Systems.
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Once thought rare, hemochromatosis is now acknowledged as one of the commonest inherited disorders, affecting one in two hundred people of western Caucasian descent. It is estimated to affect over one million people in the USA alone. This is the most comprehensive clinical reference yet on hemochromatosis. The international author team includes specialists in internal medicine, hematology, hepatology, genetics, biochemistry and molecular biology, and the contents cover all aspects of pathophysiology, epidemiology, diagnosis and treatment. The latest developments in the genetics of the disorder are well explained, and there are sections on screening, diagnostic techniques, and clinical complications. Social and ethical issues are also considered. Highly illustrated, up-to-date and authoritative, this is the definitive resource for all clinicians involved in the management of hemochromatosis, and will also be invaluable to scientists interested in iron metabolism and iron overload.
Evil Wizards! Black Magic! Betrayed Queens! Conniving Husbands! Book 3 of The Princess Maura Tales—a five book saga. If you love sword/sorcery and action/adventure stories, Wall Of Glory should be your next read. Empress Maura is a fugitive on the run. She has escaped the confines of the palace at a great cost in lives, but her escape is paramount. Everywhere, Bhuttanian soldiers search for her, but she must elude capture and forced return to her dashing, but deceitful husband Dorak, Aga of the Bhuttanians. Maura truly loves Dorak. He was everything a woman could want––handsome, cultured, witty, and courageous. He lacks only kindness. Maura couldn’t dampen the raging ambition inside ...
Extensively revised and updated, the new edition of AIDS and Other Manifestations of HIV Infection is an essential reference resource providing a comprehensive overview of the biological properties of this etiologic viral agent, its clinicopathological manifestations, the epidemiology of its infection, and present and future therapeutic options. - Expanded section on clinical manifestations includes new chapters on cardiovascular, renal and dermatologic manifestations of HIV infection - Additional chapters on molecular diagnostic techniques, the role of host genetic variation in HIV infection and its manifestations, the discovery and development of new HIV medicines, analysis of HIV dynamics using mathematical models, toxicities of antiretroviral therapy, HIV drug susceptibility testing, practical therapeutics and the global impact of HIV and AIDS