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Cell Viability Assays
This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to ...
Synaptic Vesicles
This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
Reichen & Robert
Relatively few French architects have successfully secured a position for themselves in international architecture, yet Bernard Reichen and Philippe Robert have realised projects in Greece, in Australia, in the USA, in Qatar since they founded their office in 1973. They have made a name for themselves with their conversions of 19th century buildings such as the Grande Halle de la Villette, and the Pavillon de l ́Arsenal in Paris, and increasingly issues deriving from urban development and landscape planning are featuring in their work. This book opens with an interview of Bernard Reichen and Philippe Robert which reveals their approach to architecture and urban design. In the main part of the book some 80 buildings and projects are presented and documented, including the development of the plans of one of the sites of the 2004 Olympic Games in Athens and the headquaters of Nestle France in a former chocolate factory.
Glass Structures
Flat glass opens up more possibilities for the planner than virtually any other material. Because of the technological complexity of using it, however, no specific structural forms have been developed for glass supporting frameworks as they have been for wood, concrete, and steel. This book is thus the first to present a coherent guide to the planning and design of glass supporting frameworks. The focus is on the pressure-resistant, flat supporting element as a basic building block for broad supporting structures. The spatial and constructive forms of multifunctional, self-supporting glass envelopes are vividly illustrated and systematically explained. The constructions presented exhibit new aesthetic qualities, based not on the dictum of "dematerialization" but on the poetry of gleaming and transparent planes. They ring in a new chapter in the history of glass architecture.
Imaging Dopamine
An illustrated biography of the dopamine molecule, with each chapter presenting a specific stage in the biochemical pathway for dopamine.
Shifting Baselines of Europe
This book opens the often narrow discourse on the future of Europe and criticises the false dichotomy between nationalism on the one hand and a neoliberal version of Europe on the other. Existing emancipatory projects from across the continent are presented together with reflections on strategies to achieve a democratic Europe beyond the nation state: from the municipal level to the level of transnational media, from technology and counter-surveillance to the systemic change provided by the commons movement and more. The shift towards a new way of thinking and doing politics is possible! With contributions by Etienne Balibar, Ulrike Guérot, Gesine Schwan, Renata Avila, Barbara Spinelli, Andreas Karitzis, Lorenzo Marsili, Jonas Staal, among others, and interviews with city governors from Madrid to Naples.
Introduction to Secure Outsourcing Computation
With the rapid development of cloud computing, the enterprises and individuals can outsource their sensitive data into the cloud server where they can enjoy high quality data storage and computing services in a ubiquitous manner. This is known as the outsourcing computation paradigm. Recently, the problem for securely outsourcing various expensive computations or storage has attracted considerable attention in the academic community. In this book, we focus on the latest technologies and applications of secure outsourcing computations. Specially, we introduce the state-of-the-art research for secure outsourcing some specific functions such as scientific computations, cryptographic basic operations, and verifiable large database with update. The constructions for specific functions use various design tricks and thus result in very efficient protocols for real-world applications. The topic of outsourcing computation is a hot research issue nowadays. Thus, this book will be beneficial to academic researchers in the field of cloud computing and big data security.
Synaptic Vesicles
This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
