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Single-Molecule Fluorescence Spectroscopy of Molecular Machines
This book provides a comprehensive overview of the field of single-molecule fluorescence microscopy and spectroscopy of molecular machines. It is addressed to both physical scientists (who are familiar with the fluorescence methodology but unfamiliar with the biological molecules, mechanisms and questions) and life scientists (who are familiar with the biology but unfamiliar with the large variety of available fluorescence methods). This is possible due to the background of the two authors, Achillefs Kapanidis (ANK) (biochemist) and Mike Heilemann (MH) (biophysicist). The book does not try to address the huge number of applications in non-biological areas; instead it focuses on many celebrated applications of single-molecule fluorescence on molecular machines and functional studies, with special emphasis on real-time studies of kinetics and mechanisms.
Fluorescent Methods to Study Biological Membranes
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model me...
Malaria Immunology
This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Malaria Immunology: Targeting the Surface of Infected Erythrocytes aims to be a useful and practical guide to researches to help further their study in this field. Chapter Analysis of var gene transcription pattern using DBLα-tags [Chapter 14] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Selective Autophagy
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Fluorescence Spectroscopy and Microscopy in Biology
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
