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Molecular Methods in Plant Disease Diagnostics
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Detection and Diagnostics of Plant Pathogens
This book is part of the Plant Pathology in the 21st Century Series, started in the occasion of the IX International Congress of Plant Pathology, Torino, 2008. In conjuction with the Xth International Congress of Plant Pathology, held in Beijing in August 2013. Although deriving from a Congress, the book will not have the format of traditional Proceedings, but will be organized as a resource book. It will be based on invited lectures presented at the Congress as well as by other chapters selected by the editors among offered papers. This book will cover a topic very important in the field of plant pathology, dealing with detection and diagnostics. This field of research is continuously moving forwards, due to innovation in techniques. The application of new detection and diagnostic technologies are relevant to many applied fields in agriculture. The different chapters will provide a very complete figure of the topic, from general and basic aspects to practical aspects.
Plant Virology Protocols
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
Genome Invading RNA Networks
A new paradigmatic understanding of evolution, genetic novelty, code-generating, genome-formatting factors, infectious RNA Networks, viruses and other natural genetic content operators.
The Springer Index of Viruses
The continuous growth of knowledege makes it very difficult for scientists to retrieve comprehensive and accurate data on viruses. The desired information is often dispersed in a variety of books, journals and online resources. This encyclopedia presents the latest facts about all known viruses in a standardized form created by hundreds of the world's leading virologists. Virus taxonomy represents the basic framework that allows an understanding of the complex evolutionary process that continuously takes place among viruses and their hosts. Each of the 300 taxonomically ordered chapters includes detailed information on individual genus members, historical events, the hosts they can affect (a...
Phytoplasmas
This book tries to examine all aspects related to phytoplasmas, their plant hosts and insect vectors. The opening chapter is followed by chapters on sequencing and functional genomics, which relies heavily on comparing phytoplasma genomics with that of other known bacteria. Three chapters take different approaches to differentiation, classification and taxonomy. The first group of chapters relates to aspects of phytoplasmas in plants. The first of these chapters examines the movement of phytoplasmas within the plant and the development of disease. Then look at the biochemical changes precipitated by the replication of the phytoplasma in plants, and finally at aspects of plant resistance. Chapters on the epidemiology of disease in grasses and grapevines delve into the disease process in plants. The last of the plant-related chapters examines epidemiological systems with multiple host plants. It starts with a chapter describing general aspects of insect vectors and their control, followed by an in-depth examination of the psyllid vectors and their control. It concludes with an examination of the distribution and potential spread of phytoplasma diseases and vectors worldwide.
Handbook of Molecular Microbial Ecology II
The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the fi...
