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In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Volume 1 of a series of books intended to help students of medicine, veterinary science, dentistry and biomedical science interpret the latest findings in haematology, provide a backdrop for future advances and provide more focused and in-depth reading for those wishing to follow a laboratory or clinically-based career. Each of the contributions is written by an authority in the field and is information rather than reference rich, providing an up-to-date resume of the latest advances in the subject and a focus for further reading.
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Provides an introduction to the use of radioactivity in the bioscience laboratory. The text covers general aspects of radioactivity, methods for the detection of radioactivity, radioisotope protocols used to study key cellular processes, and a summary of legislative requirements in the US and European Union. Guidance on safe handling and detailed recipes are provided.
This book explores the notion of home in the wake of the so-called refugee crisis, and asks how home and belonging can be rethought through the act of creative practices and collective writing with refugees and asylum seekers. Where Giorgio Agamben calls the refugee ‘the figure of our time’, this study places the question of home among those who experience its ruptures. Veering away from treating the refugee as a conceptual figure, the lived experiences and creative expressions of seeking asylum in Denmark and the United Kingdom are explored instead. The study produces a theoretical framework around home by drawing from a cross-disciplinary field of existential and political philosophy, narratology, performance studies and anthropology. Moreover, it argues that theatre studies is uniquely positioned to understand the performative and storied aspects of seeking asylum and the compromises of belonging made through the asylum process.
This book provides an example of the successful and rapid expansion of bioengineering within the world of the science. It includes a core of studies on bioengineering technology applications so important that their progress is expected to improve both human health and ecosystem. These studies provide an important update on technology and achievements in molecular and cellular engineering as well as in the relatively new field of environmental bioengineering. The book will hopefully attract the interest of not only the bioengineers, researchers or professionals, but also of everyone who appreciates life and environmental sciences.
Now in a fully revised and updated fourth edition, Science and Soccer is still the most comprehensive and accessible introduction to the physiology, biomechanics and psychology behind the world's most popular sport. Offering important guidance on how science translates into practice, the book examines every key facet of the sport, with a particular focus on the development of expert performers. The topics covered include: • anatomy, physiology, psychology; sociology and biomechanics; • principles of training; • nutrition; • physical and mental preparation; • playing surfaces and equipment; injury • decision-making and skill acquisition; • coaching and coach education; • performance analysis; • talent identification and youth development. Science and Soccer: Developing Elite Performers is a unique resource for students and academics working in sports science. It is essential reading for all professional support staff working in the game, including coaches at all levels, physiotherapists, conditioning specialists, performance analysts, club doctors and sport psychologists.
A review of recent developments in polypeptide and protein chemistry and biology relevant to their exploitation as pharmaceuticals. Topics covered include the structure and folding of proteins, polypeptide function, the current status of polypeptide pharmaceuticals, quality control and safety.
In recent decades, the sporting landscape has seen many changes, notably the inclusion and professionalization of sport science and coaching. With this change has come significant demand for applied research surrounding soccer science and performance coaching, leading to new theory and methodology across all aspects of the game. This book brings together the most fundamental components of soccer science and performance coaching through modern, integrated coaching science methods implemented by leading practitioners and researchers. The expertise included in this book provides a unique blend of modern, soccer-specific research trends with innovative coaching theory, implemented at an elite le...
Immunogenicity of Biopharmaceuticals is the first book to comprehensively address the potential of an immune response to biopharmaceuticals. It is intended to give a broad overview of the current state-of-the-art regarding this subject. The chapters range from an overview of the immune system and factors that may trigger the immune system, via detection of antibodies and clinical implications, to various case examples and the regulatory view on immunogenicity.