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Methods for Fragments Screening Using Surface Plasmon Resonance
This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
Major Companies of the Arab World 1993/94
This book represents the seventeenth edition of the leading IMPORTANT reference work MAJOR COMPANIES OF THE ARAB WORLD. All company entries have been entered in MAJOR COMPANIES OF THE ARAB WORLD absolutely free of ThiS volume has been completely updated compared to last charge, thus ensuring a totally objective approach to the year's edition. Many new companies have also been included information given. this year. Whilst the publishers have made every effort to ensure that the information in this book was correct at the time of press, no The publishers remain confident that MAJOR COMPANIES responsibility or liability can be accepted for any errors or OF THE ARAB WORLD contains more informati...
Cleaning Pakistan’s Air
The harm to Pakistanis’ health, economy, and environment from urban air pollution is among the highest in South Asia, exceeding several high-profile causes of mortality and morbidity in Pakistan. This report details a broad spectrum of research on Pakistan’s air quality management challenges and presents concrete steps to achieve improvements.
Playing It My Way
'I don't think anyone, apart from Don Bradman, is in the same class as Sachin Tendulkar.' -Shane Warne Readers are in love with Sachin Tendulkar's autobiography: 'A must read for anyone who knows cricket' ⭐⭐⭐⭐⭐ 'An idol . . . An inspiration' ⭐⭐⭐⭐⭐ 'A great book by one of the all time greats' ⭐⭐⭐⭐⭐ 'Brings back so many wonderful memories'⭐⭐⭐⭐⭐ 'This book has made me feel proud to be a lover of the game and has inspired me to succeed in everything I do.' ⭐⭐⭐⭐⭐ The story of the greatest cricket player of all time, told in his own words. __________ The greatest run-scorer in the history of cricket, Sachin Tendulkar retired in 2013 after an astoni...
Intelligent Technologies and Applications
This book constitutes the refereed proceedings of the Second International Conference on Intelligent Technologies and Applications, INTAP 2019, held in Bahawalpur, Pakistan, in November 2019. The 60 revised full papers and 6 revised short papers presented were carefully reviewed and selected from 224 submissions. Additionally, the volume presents 1 invited paper. The papers of this volume are organized in topical sections on AI and health; sentiment analysis; intelligent applications; social media analytics; business intelligence;Natural Language Processing; information extraction; machine learning; smart systems; semantic web; decision support systems; image analysis; automated software engineering.
Kousarnag: Journey to the majestic lake
The complete story of the trekking
A Bad Beginning
Now in his 80s, Gai Eaton describes how, after a strange childhood completely isolated from other children, followed by a Cambridge education and life as an actor and later as a diplomat, circumstances led him at the age of 30 to Islam.Fascinated by the vagaries of human behavior and the strangeness of human destinies, he has observed the human scene with a novelist's eye and traced the profound changes in attitudes and tastes which have taken place in a single lifetime. He recounts his youthful adventures with the clear-sight and understanding only possible for someone whom age has freed from the passions which once possessed him. What makes this work unique is the juxtaposition of hindsight with diary entries made at the time, which gives a quality of immediacy to a true story that includes reminiscences of the diplomatic life and an outline of the Sufi path.
Captain Cool
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The Medical Register
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