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This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
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Updated for 1997 with coverage of new technologies like Java, ActiveX, teleconferencing, 3D chat, Internet Explorer 3 and Netscape Navigator 3, this guide contains everything that the reader needs to learn to completely master the Internet. The CD includes all the software the user needs to get connected and on the Internet, regardless of the computing platform, and the entire book in HTML format for easy browsing and links to online resources.
In this book a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion. They further provide methods that present classic and state-of-the-art experimental approaches in a variety of systems.
Finally, we further explore the role of daf-12 on lifespan and thermotolerance, as well as examine the effects of environmental perturbation on the lifespan of germline deficient animals.