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This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cancer Cell Biology: Methods and Protocols aims to provide a comprehensive set of tools for the analysis of cancer cell biology in the lab.
Monica Harris-Day's perfect world begins a downward spiral the afternoon she comes home to find her husband in bed . . . with another man. After confronting Kevin, her husband of two years, Monica discovers he's had a lifelong struggle with homosexuality that began at the age of ten after he was molested by a deacon in the church. For years, Kevin has sought deliverance, crying out to God to make him straight. He explains his deceit by saying that he thought he had truly been delivered when he married Monica, but was afraid to share his past with her for fear she wouldn't marry him. Kevin begs Monica's forgiveness and wants to save their marriage. He is convinced that God has indeed delivere...
Revealing essential roles of the tumor microenvironment in cancer progression, this volume focuses on non-hematopoietic cells within the tumor microenvironment.Further, it teaches readers about the roles of distinct constituents of the tumor microenvironment and how they affect cancer development. Topics include fibroblasts, adipocytes, mesenchymal stem cells, stellate cells, and more. Taken alongside its companion volumes, Tumor Microenvironment: Non-Hematopoietic Cells updates us on what we know about the different aspects of the tumor microenvironment as well as future directions. Useful for introducing the newer generation of researchers to the history of how scientists focused in the tumor microenvironment and how this knowledge is currently applied for cancer treatments, it will be essential reading for advanced cell biology and cancer biology students as well as researchers seeking an update on research in the tumor microenvironment. All of the chapter authors are renowned international experts in the cancer biology field in specific subfields that will be the focus of their chapters.
The American Heart Association’s Scientific Sessions 2016 is bringing big science, big technology, and big networking opportunities to New Orleans, Louisiana this November. This event features five days of the best in science and cardiovascular clinical practice covering all aspects of basic, clinical, population and translational content.
Faculties, publications and doctoral theses in departments or divisions of chemistry, chemical engineering, biochemistry and pharmaceutical and/or medicinal chemistry at universities in the United States and Canada.
All Devon Wright wants is peace and some time to chill with his boys. He's jerseyed up and waiting for his boy, Charles, to show up with tickets to the Atlanta Falcons' season opener. Instead, at his front door he finds "Satan's little sister," aka Quartisha Shauntae Randall, his baby momma. Once again, she's dropping off their daughter unexpectedly and ruining his plans. Devon doesn't mind, though. His daughter, Brianna, is the most important thing in his life. He's worried about the poor example Shauntae (He refuses to EVER call her by her first name) is setting with her ghetto-fabulous lifestyle. He can't believe he ended up with such a crazy baby momma. He's vowed to stay away from all women for a while—until Charles introduces him to Cassandra. Cassandra is nothing like Shauntae. She's a good, church-going woman. Devon is pleasantly surprised to find out that Cassandra's not what he thought a Christian woman would be. Cassandra Parker is not only fine; she's also smart, sassy, and sexy. Devon is intrigued, and he wants to get to know Cassandra better, but how will he be able to handle his daughter, his crazy baby momma, and a new woman in his life?
A national bestselling author draws readers into a funny yet inspiring novel about three close friends searching for their own Mr. Rights, while trying to have trust and faith in God to send them each a soul mate. Original.