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In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Catalogus met meer dan honderd hedendaagse kunstenaars die deelnamen aan de twaalfde Documenta in Kassel, een vijfjaarlijkse tentoonstelling gewijd aan moderne kunst.
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This updated second edition of Acute Ischemic Stroke: Imaging and Intervention provides a comprehensive account of the state of the art in the diagnosis and treatment of acute ischemic stroke. The basic format of the first edition has been retained, with sections on fundamentals such as pathophysiology and causes, imaging techniques and interventions. However, each chapter has been revised to reflect the important recent progress in advanced neuroimaging and the use of interventional tools. In addition, a new chapter is included on the classification instruments for ischemic stroke and their use in predicting outcomes and therapeutic triage. All of the authors are internationally recognized experts and members of the interdisciplinary stroke team at the Massachusetts General Hospital and Harvard Medical School. The text is supported by numerous informative illustrations, and ease of reference is ensured through the inclusion of suitable tables. This book will serve as a unique source of up-to-date information for neurologists, emergency physicians, radiologists and other health care providers who care for the patient with acute ischemic stroke.
The documenta 12 magazines are a collective worldwide editorial project. In advance of the exhibition in Kassel, documenta 12 magazines have opened up a central site for reflection and visual contemplation. Since early 2006, influential journals in the realm of art, as well as specialist publications operating in discursive fields beyond the major art centres, have been publishing and discussing contributions essays, interviews, photo reportages, features, interventions from artists and articles of fiction in relation to documenta 12's three main themes: Is modernity our antiquity? What is bare life? What is to be done? This Book is a compilation of all three magazines.
The 25 contributions of this volume represent a selection from the more than 120 papers originally presented at the International Conference on Multilingual Individuals and Multilingual Societies (MIMS), held in Hamburg (October 2010) and organized by the Collaborative Research Center Multilingualism after twelve years of successful research. It presents a panorama of contemporary research in multilingualism covering three fields of investigation: (1) the simultaneous and successive acquisition of more than one language, including language attrition in multilingual settings, (2) historical aspects of multilingualism and variance, and (3) multilingual communication. The papers cover a vast variety of linguistic phenomena including morphology, syntax, segmental and prosodic phonology as well as discourse production and language use, taking both individual and societal aspects of multilingualism into account. The languages addressed include numerous Romance, Slavic and Germanic varieties as well as Welsh, Hungarian, Turkish, and several South African autochthonous languages.
Now in its revised, updated Second Edition, this pocket-sized handbook is a practical quick-reference guide to the diagnosis and management of neurologic diseases. It presents specific management recommendations in a succinct outline format and includes protocols, step-by-step tests and procedures, and treatment algorithms. This handbook is unique in its inclusion of material from related disciplines such as general medicine, cardiology, psychiatry, neurosurgery, neuroanatomy, and radiology. The authors offer guidance in using contemporary neuroimaging techniques in diagnosis.