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Quantitation of mRNA by Polymerase Chain Reaction
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Documenta 12
Catalogus met meer dan honderd hedendaagse kunstenaars die deelnamen aan de twaalfde Documenta in Kassel, een vijfjaarlijkse tentoonstelling gewijd aan moderne kunst.
Stroke Genetics
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Universität Leipzig
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Documenta magazine
The documenta 12 magazines are a collective worldwide editorial project. In advance of the exhibition in Kassel, documenta 12 magazines have opened up a central site for reflection and visual contemplation. Since early 2006, influential journals in the realm of art, as well as specialist publications operating in discursive fields beyond the major art centres, have been publishing and discussing contributions essays, interviews, photo reportages, features, interventions from artists and articles of fiction in relation to documenta 12's three main themes: Is modernity our antiquity? What is bare life? What is to be done? This Book is a compilation of all three magazines.
Acute Ischemic Stroke
This updated second edition of Acute Ischemic Stroke: Imaging and Intervention provides a comprehensive account of the state of the art in the diagnosis and treatment of acute ischemic stroke. The basic format of the first edition has been retained, with sections on fundamentals such as pathophysiology and causes, imaging techniques and interventions. However, each chapter has been revised to reflect the important recent progress in advanced neuroimaging and the use of interventional tools. In addition, a new chapter is included on the classification instruments for ischemic stroke and their use in predicting outcomes and therapeutic triage. All of the authors are internationally recognized experts and members of the interdisciplinary stroke team at the Massachusetts General Hospital and Harvard Medical School. The text is supported by numerous informative illustrations, and ease of reference is ensured through the inclusion of suitable tables. This book will serve as a unique source of up-to-date information for neurologists, emergency physicians, radiologists and other health care providers who care for the patient with acute ischemic stroke.
Digital Privacy
This book documents the R&D outcome of the PRIME Project, an R&D project partially funded by the European Union’s Sixth Framework Programme and the Swiss Federal Office for Education and Science. PRIME has focused on privacy-enhancing identity management techniques and systems to support users’ sovereignty over their personal privacy and enterprises’ privacy-compliant data processing. During the course of four years, the project has involved over a hundred researchers and professionals from 22 major European academic and industrial organizations conducting R&D work in areas relevant to digital privacy. The book presents 28 detailed chapters organized in five parts: Introductory summary, legal, social, and economic aspects, realization of privacy-enhancing user-centric identity management, exploitation of PRIME results for applications, conclusions drawn and an outlook on future work. As the first coherent presentation of the topic, this book will serve as a valuable source of reference and inspiration for anybody working on digital privacy.
