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Membrane proteins are essential determinants of many biological processes in plants. They function in metabolic processes, signal transduction, transport of small molecules and polymers across endo- and plasma membranes, and intercompartmental trafficking of proteins, lipids, and cell wall components. During these integrative processes, dynamic interactions of membrane proteins with other membrane or soluble components are thought to provide a high degree of flexibility that usually characterizes higher plants. This concept is supported by the recent release of a first, partial Arabidopsis interactome by the Arabidopsis Interactome Mapping Consortium (http://www.sciencemag.org/content/333/6042/601.full.htm). The Arabidopsis interactome reveals a strong enrichment of a few network communities, including those for transmembrane transport and vesicle trafficking. Strikingly, the large transmembrane transport community shares a high amount of proteins with the vesicle trafficking community suggesting a strong physical and functional overlap and interaction.
Plants in tropical regions are coping with enormous challenges of physiological stresses owing to changing environmental and climatic conditions. Rapid growth of human population and rampant exploitation of fossil fuels and other developmental activities are actively contributing to such perturbations. The Intergovernmental Panel on Climate Change has projected a sustained increase in carbon dioxide (CO2) emissions and thereby a rise in global temperature in the coming decades. The resultant changes in precipitation patterns are now evident across the globe due to intensication of hydrological cycle. Moreover, gaseous and particulate pollutants are also an immense challenge for tropical plan...
The decade since the publication of the third edition of this volume has been an era of great progress in biology in general and the plant sciences in particular. This is especially true with the advancements brought on by the sequencing of whole genomes of model organisms and the development of "omics" techniques. This fourth edition of Plant Roots: The Hidden Half reflects these developments that have transformed not only the field of biology, but also the many facets of root science. Highlights of this new edition include: The basics of root research and their evolution and role in the global context of soil development and atmosphere composition New understandings about roots gained in t...
This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.
Beards and Texts explores the literary portrayal of beards in medieval German texts from the mid-twelfth to the early sixteenth centuries. It argues that as the pre-eminent symbol for masculinity the beard played a distinctive role throughout the Middle Ages in literary discussions of such major themes as majesty and humanity. At the same time beards served as an important point of reference in didactic poetry concerned with wisdom, teaching and learning, and in comedic texts that were designed to make their audiences laugh, not least by submitting various figure-types to the indignity of having their beards manhandled. Four main chapters each offer a reading of a work or poetic tradition of...
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes. - First book on the GUS reporter system - Up-to-date reference lists following each of the fourteen chapters - Comb-bound for convenient bench-top use - Written by leading authorities including R.A. Jefferson, inventor of the GUS assay - Illustrated with color - GUS detection by fluorometric, spectrophotometric, and histochemical methods - Fast, automated assays
The importance of the plant growth regulator auxin for plant growth has long been recognized, even before the discovery of its chemical structures in the early 20th century. Physiological studies in the decades since have demonstrated that auxin is unidirectionally transported in plants, a process dubbed polar auxin transport. It is the polar auxin transport process that generates a local auxin concentration gradient and regulates a broad array of physiological and developmental processes. The discoveries of auxin transport carrier proteins that mediate auxin influx into and efflux out of transport-competent cells and auxin receptor proteins for auxin signaling in the last few decades repres...
This book presents recent advances in understanding the physiological and molecular mechanisms of different abiotic stresses such as high or low temperature, salinity, drought, flooding, soil acidity, heavy metals, light stress and ozone stress, and discusses the multifaceted role of phytohormones in stress adaptation and the underlying mechanisms. Aimed at students and researchers in the field of plant science, it offers a comprehensive overview of the versatile roles and interactions of different phytohormones in response to a specific stress factor and examines the possible physiological and molecular mechanisms that have been the subject of recent research.