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Fish Development and Genetics
The zebrafish is the most important fish model in developmental andgenetic analyses. This book contains 19 review articles covering abroad spectrum of topics, from development to genetic tools. Thecontents range from early development, the role of maternal factorsand gastrulation, to tissue differentiation and organogenesis, such asdevelopment of the organizer, notochord, floor plate, nervous system, somites, muscle, skeleton and endoderm
The Zebrafish: Genetics, Genomics and Informatics
This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to the zebrafish community.* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Illustrates many techiques in full-color * Summarizes the Zebrafish Genome Project
Cytoskeleton
The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with "motility" that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer.
Essential Zebrafish Methods: Genetics and Genomics
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where rel...
Zebrafish
The zebrafish has become one of the most important model organisms to study biologial processes in vivo. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. The next few years will see the completion of large scale initiatives that exploit the zebrafish as a model system for the understanding of gene function in vertebrates, including the sequencing of the genome. The zebrafish will therefore play an increasingly important role in the future of biomedical research. Whole genome sequencing projects, such as the human genome project, have led to th...
The Zebrafish: Genetics and Genomics
This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites* Relevant to clinicians interested in vertebrate models of human congenital diseases
The Zebrafish: Genetics, Genomics and Informatics
This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.
Insights from Animal Reproduction
The chapters in this volume of "Insights from Animal Reproduction" address several, particular hot topics in the field of reproduction. The book begins with a comprehensive overview of the cryopreservation of sheep-produced embryos. The following chapter revises the assisted reproductive techniques available for South American wild mammals. Chapter 3 presents the technical procedures necessary to produce transgenic goats. Chapter 4 provides a comprehensive revision of the major molecular determinants of litter size in prolific species. Chapter 5 examines the germ cell determinant transmission, segregation, and function using the zebrafish as a model for germ cell specification in the embryo....
