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Pseudomonas Aeruginosa Lectin LecB Impairs Keratinocyte Fitness by Abrogating Growth Factor Signalling
Abstract: Lectins are glycan-binding proteins with no catalytic activity and ubiquitously expressed in nature. Numerous bacteria use lectins to efficiently bind to epithelia, thus facilitating tissue colonisation. Wounded skin is one of the preferred niches for Pseudomonas aeruginosa, which has developed diverse strategies to impair tissue repair processes and promote infection. Here, we analyse the effect of the P. aeruginosa fucose-binding lectin LecB on human keratinocytes and demonstrate that it triggers events in the host, upon binding to fucosylated residues on cell membrane receptors, which extend beyond its role as an adhesion molecule. We found that LecB associates with insulin-like...
A Label-free Optical Detection of Pathogens in Isopropanol as a First Step Towards Real-time Infection Prevention
Abstract: The detection of pathogens is a major public health issue. Every year, thousands of people die because of nosocomial infections. It is therefore important to be able to detect possible outbreaks as early as possible, especially in the hospital environment. Various pathogen detection techniques have already been demonstrated. However, most of them require expensive and specific equipment, and/or complex protocols, which, most of the time, involve biochemical reaction and labelling steps. In this paper, a new method that combines microscopic imaging and machine learning is described. The main benefits of this approach are to be low-cost, label-free and easy to integrate in any suitable medical device, such as hand hygiene dispensers. The suitability of this pathogen detection method is validated using four bacteria, both in PBS (Phosphate Buffered Saline) and in isopropanol. In particular, we demonstrated an efficient pathogenic detection that is sensible to changes in the composition of a mixture of pathogens, even in alcohol-based solutions
Julie Davidson
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Julie Christie
Traces the life and career of the popular British actress, explains how she got her start in acting, and discusses each of her film roles
An Julie
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Times Past
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Band of Gold and Other Stories
This second book in the Kaleidoscope series brings another stunning collection of carefully selected heart-warming and emotional short stories. All you have to do is turn the page and be instantly whisked away into another dimension. Will Kate's wish ever come true? Why is Jorja determined to...? Can Aerona fulfil her pledge? Two diaries, two women, a lifetime apart Circle the world like the ever changing pattern of the Kaleidoscope, with this exciting new series from historical author Julie Roberts
