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Manipulating the Mouse Embryo
Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.
Mouse Phenotypes
The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step–by–step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects.
Mouse Phenotypes
"The laboratory mouse is an important model in biomedical research. This volume provides the concepts and tools needed to generate mutant mice and analyze their phenotypes"--
The Genetics and Biology of Sex Determination
Nature employs a wide variety of sex determining mechanisms and it is only comparatively recently that the tools have become available for these to be explored at the cellular and molecular levels. A major landmark was the discovery in 1990 of the SRY gene and the subsequent demonstration of its key role in triggering male sex determination in transgenic mice. This book reviews and discusses our current understanding of the molecular genetic pathways of sex determination, with special emphasis on vertebrates. It features comparisons with other modes of sex determination, consideration of the biology of sexual development and discussion of the evolution of sex-determining mechanisms. By bringing together an international and interdisciplinary group of experts who study many different aspects of the problem, the book highlights much new and exciting work in this area and serves to identify and stimulate promising new research directions.
Bat Evolution, Ecology, and Conservation
Recent advances in the study of bats have changed the way we understand this illusive group of mammals. This volume consist of 25 chapters and 57 authors from around the globe all writing on the most recent finding on the evolution, ecology and conservation of bats. The chapters in this book are not intended to be exhaustive literature reviews, but instead extended manuscripts that bring new and fresh perspectives. Many chapters consist of previously unpublished data and are repetitive of new insights and understanding in bat evolution, ecology and conservation. All chapters were peer-reviewed and revised by the authors. Many of the chapters are multi-authored to provide comprehensive and authoritative coverage of the topics.
Mouse Phenotypes
"The laboratory mouse is an important model in biomedical research. This volume provides the concepts and tools needed to generate mutant mice and analyze their phenotypes"--
Transgenesis
This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Why Study Biology by the Sea?
"Since the middle of the 19th century, biologists have migrated to the seashore to study marine organisms as a way of understanding life. By the turn of the 20th century, such work was being done inside permanent seaside field stations. The Stazione Zoologica, in Naples, Italy (from 1874), and the Marine Biological Laboratory, in Woods Hole, Massachusetts (from 1888), attracted leaders in many biological fields, and helped establish biology as a modern science. Why Study Biology by the Sea? tells the story of these unique scientific institutions while attempting to answer the contemporary question, "Why study biology by the sea?" The volume examines the origins and value of these places via perspectives that range from cell biology to philosophy of science"--
