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Techniques for Biochemical Analysis
Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are...
Advanced Methods in Molecular Biology and Biotechnology
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, prov...
Landmark Experiments in Molecular Biology
Landmark Experiments in Molecular Biology critically considers breakthrough experiments that have constituted major turning points in the birth and evolution of molecular biology. These experiments laid the foundations to molecular biology by uncovering the major players in the machinery of inheritance and biological information handling such as DNA, RNA, ribosomes, and proteins. Landmark Experiments in Molecular Biology combines an historical survey of the development of ideas, theories, and profiles of leading scientists with detailed scientific and technical analysis. - Includes detailed analysis of classically designed and executed experiments - Incorporates technical and scientific analysis along with historical background for a robust understanding of molecular biology discoveries - Provides critical analysis of the history of molecular biology to inform the future of scientific discovery - Examines the machinery of inheritance and biological information handling
Essentials of Pharmaceutical Analysis
Recent advances in the pharmaceutical sciences and biotechnology have facilitated the production, design, formulation and use of various types of pharmaceuticals and biopharmaceuticals. This book provides detailed information on the background, basic principles, and components of techniques used for the analysis of pharmaceuticals and biopharmaceuticals. Focusing on those analytical techniques that are most frequently used for pharmaceuticals, it classifies them into three major sections and 19 chapters, each of which discusses a respective technique in detail. Chiefly intended for graduate students in the pharmaceutical sciences, the book will familiarize them with the components, working principles and practical applications of these indispensable analytical techniques.
Processes in Microbial Ecology
Microbial ecology is the study of interactions among microbes in natural environments and their roles in biogeochemical cycles, food web dynamics, and the evolution of life. Microbes are the most numerous organisms in the biosphere and mediate many critical reactions in elemental cycles and biogeochemical reactions. Because microbes are essential players in the carbon cycle and related processes, microbial ecology is a vital science for understanding the role of the biosphere in global warming and the response of natural ecosystems to climate change. This novel textbook discusses the major processes carried out by viruses, bacteria, fungi, protozoa and other protists - the microbes - in fres...
The Occupied Clinic
In The Occupied Clinic, Saiba Varma explores the psychological, ontological, and political entanglements between medicine and violence in Indian-controlled Kashmir—the world's most densely militarized place. Into a long history of occupations, insurgencies, suppressions, natural disasters, and a crisis of public health infrastructure come interventions in human distress, especially those of doctors and humanitarians, who struggle against an epidemic: more than sixty percent of the civilian population suffers from depression, anxiety, PTSD, or acute stress. Drawing on encounters between medical providers and patients in an array of settings, Varma reveals how colonization is embodied and ho...
Introduction to Plant Tissue Culture
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
Strawberry
Methods of strawberry cultivation have undergone extensive modification and this book provides an up-to-date, broad and balanced scientific review of current research and emerging challenges. Subjects covered range from plant propagation, architecture, genetic resources, breeding, abiotic stresses and climate change, to evolving diseases and their control. These topics are examined in three sections: 1. Genetics, Breeding and Omics - covering genetic resources, breeding, metabolomics, transcriptomics, and genetic transformation of strawberry. 2. Cultivation Systems and Propagation - discusses plant architecture, replanting problems and plant propagation techniques. 3. Disease and Stress Mana...
Vegetable Grafting
This book provides comprehensive and current scientific and practical knowledge on vegetable grafting, a method gaining considerable interest as an alternative to the use of fumigants to protect crops from soil-borne diseases.
English Education in India, 1715-1835
This book identifies and describes the first stage in the advent and growth of English education in India. The first schools in India were the charity schools, asylums and orphanages opened under the auspices of the Church of England for religious instruction, training and care of ‘half-caste’ or mixed-race children, the progeny of Protestant fathers from Indian women. It examines the influence of the ‘half-caste’ community and the missionaries on the growing Indian demand for English education and opportunities for employment. The well-entrenched scenarios on the pre-history of Hindoo College Calcutta are re-examined in the light of new evidence discussed here for the first time. The book further analyses the shifts in the educational policies by the British colonial administrators and the interventions by the likes of Trevelyan, Macaulay and Bentinck. Detailed and insightful, this volume will be of great interest to students and researchers of history, literature, postcolonial studies, cultural studies, colonial expansion, and South Asian studies.
