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I'm A Quantum Dot Chemist Now!
What is a quantum dot chemist? What does he or she do?Meet Sandy, a kid like you and me, who grew up wanting to become a school teacher. Then she discovered she loved doing chemistry. Now, she does chemistry research and teaches chemistry to college students. Learn how Sandy discovered a quantum dot that emits white light and how she is working to use quantum dots to track the movement of medicines in brains. And learn how Sandy suffered from seasonal bipolar disorder and then, with help from doctors, learned how to manage her brain health and thrive in her career and as a mother.The Who Me? series features inspirational biographies of scientists who young readers will be able to identify wi...
I'm An Immunologist Now!
Meet Wonder, a kid like you and me, who moved around a lot and went to lots of different schools. As a teenager, she worked as a waitress, at a library, and helped out with older people. Then, she discovered she wanted to become a doctor. Along the way, she met a patient with an illness called sarcoidosis. This patient's immune system was not working the way it was supposed to. Wonder decided that she would learn how to do medical research so that she could learn about this disease. Someday, she hopes to help find a cure for sarcoidosis.The Who Me? series features inspirational biographies of scientists whom young readers will be able to identify with as people like themselves. Written by th...
DNA Repair Protocols
The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a s...
RNA Silencing
A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
I'm A Mathematician Now!
Meet Marcelo, a kid like you and me, who grew up in Brazil wanting to become a philosopher and solve logic puzzles. Philosophy led him to physics, and physics led him to mathematics. Learn how Marcelo thinks about mathematical equations as puzzles to solve and how solving one of those puzzles helped him learn about how the universe works.The Who Me? series features inspirational biographies of scientists whom young readers will be able to identify with as people like themselves. Written by the scientists themselves and co-authors from Vanderbilt University, the books are designed to help young readers understand that scientists are regular people like themselves who are excited about learnin...
Mast Cells
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
Chemical Genomics
Chemical genomics is an exciting new field that aims to transform biolo- cal chemistry into a high-throughput industrialized process, much in the same way that molecular biology has been transformed by genomics. The inter- tion of small organic molecules with biological systems (mostly proteins) underpins drug discovery in the pharmaceutical and biotechnology industries, and therefore a volume of laboratory protocols that covers the key aspects of chemical genomics would be of use to biologists and chemists in these orga- zations. Academic scientists have been exploring the functions of proteins using small molecules as probes for many years and therefore would also b- efit from sharing idea...
Nanotechnology: Science and Computation
Nanoscale science and computing is becoming a major research area as today's scientists try to understand the processes of natural and biomolecular computing. The field is concerned with the architectures and design of molecular self-assembly, nanostructures and molecular devices, and with understanding and exploiting the computational processes of biomolecules in nature. This book offers a unique and authoritative perspective on current research in nanoscale science, engineering and computing. Leading researchers cover the topics of DNA self-assembly in two-dimensional arrays and three-dimensional structures, molecular motors, DNA word design, molecular electronics, gene assembly, surface layer protein assembly, and membrane computing. The book is suitable for academic and industrial scientists and engineers working in nanoscale science, in particular researchers engaged with the idea of computing at a molecular level.
Phosphodiesterase Methods and Protocols
Research leaders in the PDE field describe new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. Presented in step-by-step detail, these readily reproducible methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatio-temporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs.
