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Archaea
This volume provides an overview of well-established methods optimized for diverse archaeal model organisms and is a source of protocols facilitating access to the molecular and cellular biology characterization of these fascinating organisms. Chapters are divided into five parts detailing available genetic tools, molecular and cellular biology methods, strategies to study the ecophysiology of archaea, and classroom protocol. Each main thematic part is also introduced by future-oriented and authoritative primers. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Archaea: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Ribosome Biogenesis
This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of ...
Prokaryotic Gene Regulation
This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of re...
RNA Metabolism and Gene Expression in Archaea
This book focuses on the regulation of transcription and translation in Archaea and arising insights into the evolution of RNA processing pathways. From synthesis to degradation and the implications of gene expression, it presents the current state of knowledge on archaeal RNA biology in 13 chapters. Topics covered include the modification and maturation of RNAs, the function of small non-coding RNAs and the CRISPR-Cas defense system. While Archaea have long been considered exotic microbial extremophiles, they are now increasingly being recognized as important model microorganisms for the study of molecular mechanisms conserved across the three domains of life, and with regard to the relevance of similarities and differences to eukaryotes and bacteria. This unique book offers a valuable resource for all readers interested in the regulation of gene expression in Archaea and RNA metabolism in general.
Les archées, micro-organismes du troisième domaine du vivant 1
Les archées (ou Archaea) constituent un nouveau domaine du vivant au côté des bactéries et des eucaryotes. Ces micro-organismes sont uniques par leurs aspects cellulaires et moléculaires. Ils possèdent des liens évolutifs avec les premières cellules eucaryotes et sont désormais utilisés pour élucider des questions biologiques fondamentales. Championnes de l’extrêmophilie, les archées permettent de lever le voile sur les limites du vivant sur Terre. Les archées, micro-organismes du troisième domaine du vivant 1 présente la découverte et l’évolution du champ de recherche des archées. Il s’interroge sur leur histoire évolutive, leur intégration dans l’arbre du vivant et examine ce monde complexe et extrêmement diversifié au vu de leurs niches écologiques et de leur virosphère encore largement inexplorée.
Les archées, micro-organismes du troisième domaine du vivant 2
Les archées (ou Archaea) constituent un nouveau domaine du vivant au côté des bactéries et des eucaryotes. Ces micro-organismes sont uniques par leurs aspects cellulaires et moléculaires. Ils possèdent des liens évolutifs avec les premières cellules eucaryotes et sont désormais utilisés pour élucider des questions biologiques fondamentales. Championnes de l’extrêmophilie, les archées permettent de lever le voile sur les limites du vivant sur Terre. Les archées, micro-organismes du troisième domaine du vivant 2 expose les divers mécanismes moléculaires impliqués dans les processus fondamentaux du maintien des génomes et de la régulation de l’expression génique des cel...
Archaeal Virus-host Interactions
The work presented in this thesis provides novel insights in several aspects of the molecular biology of Archaea, Bacteria and their viruses. The archaeal virus Sulfolobus islandicus rod-shaped virus 2 (SIRV2), has a remarkable infection cycle. Infection with SIRV2 results in the formation of large virus associated pyramids (VAPs) on the host cell surface. The pyramids open during the final step of the infection cycle, to allow the release of virions. This virus release mechanism is unique. The VAPs are formed by self-assembly of one virus-encoded protein, PVAP. VAPs exist as discrete particles, and are baseless pyramids with heptagonal perimeter. The assembly process of the VAPs is describe...
