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Single Cell ‘Omics of Neuronal Cells
This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
Methods for Imaging Cell Membranes
· Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) · Measuring membrane protein dynamics and diffusion using fluorescence correlation spectroscopy (FCS) · Mapping membrane lipid backing using environmentally sensitive fluorescence probes · Mapping membrane thickness and rigidity using atomic force microscopy · Mapping membrane proteins and the cytoskeleton using electron microscopy
Optical Probes in Biology
Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use
Handbook of Neurophotonics
The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light. Including 32 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that...
Super-Resolution Imaging in Biomedicine
This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.
Imaging from Cells to Animals In Vivo
Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to pro...
Imaging in Photodynamic Therapy
This book covers the broad field of cellular, molecular, preclinical, and clinical imaging either associated with or combined with photodynamic therapy (PDT). It showcases how this approach is used clinically for cancer, infections, and diseases characterized by unwanted tissue such as atherosclerosis or blindness. Because the photosensitizers are also fluorescent, the book also addresses various imaging systems such as confocal microscopy and small animal imaging systems, and highlights how they have been used to follow and optimize treatment, and to answer important mechanistic questions. Chapters also discuss how imaging has made important contributions to clinical outcomes in skin, bladder, and brain cancers, as well as in the development of theranostic agents for detection and treatment of disease. This book provides a resource for physicians and research scientists in cell biology, microscopy, optics, molecular imaging, oncology, and drug discovery.
RBS Visitors Guide INDIA - Maharashtra
Maharashtra - The land of World Heritage Sites of Ajanta and Ellora in the historic city of Aurangabad, the matchless forts of Sindhdurg, and Pratapgad, the pilgrimage sites of Shirdi, Nasik, and Trimbakeshvar, amazing beauty of hill resorts of Mahabaleshwar and Lonavala, the cultural capital of Kolhapur, the virgin beaches of South Konkan, and the energetic cities of Mumbai and Pune are some of the many fascinating facets of the state. Book Contents:Introduction Maharashtra The book offers interesting insight about Maharashtra's Culture, music, dance, art and craft, fairs and festivals, Adventure and sports, wildlife and flora. The destinations are signposted with details of accessibility and connectivity, Tourist attractions,Local Directory, Guide maps, Popular and little known places of interest. The book has Listing of Hotels, Popular places of dining out, Restaurants Shopping areas, Details on tourist offices, Travel services providers,Travel tips.
Google、臉書、微軟專家教你的66堂科技趨勢必修課
北美好書獎商業類冠軍 三位矽谷專家，用零基礎就能懂的語言 將最核心的科技趨勢濃縮一冊，讓你 像賈伯斯一樣思考，秒懂科技及商業動態！ Google搜索是怎麼運作的？ Spotify如何確定推薦給你的歌曲？ 為什麼華盛頓郵報同一篇文章都有兩個版本的標題？ Facebook如何決定哪些內容會出現在你的動態消息？ 臉書為什麼要以十億美元買下IG？ 這些問題，你知道答案嗎？ 科技新知每天洪水般湧來，你是能理解吸收、跟上趨勢，在自身的工作與生活中善用，或是看不懂、想不通，平白讓職涯發展與機會溜走？ 終於等到一本書，即使...
Multiplexed Imaging
This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.
