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Principles and Practices of Unbiased Stereology
"An important book for the libraries of professionals in neurobiology, neurology, and neurosurgery." -- Doody's Book Review Service
Unbiased Stereology
Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience. To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.
Stereology and Stochastic Geometry
Somebody had to do it. The Chinese speak of deep water wells called "grandfather wells" because they take three generations of diggers to complete. Imagine the thought of such a well being abandoned incomplete by the third generation. What a loss! This book is like a grandfather well except that it has taken only two generations, John Hilliard's and mine, to finish. When I saw his manuscript lying in a heap, I decided that I must spend the time to put it and his notes into a publishable form. Now, it is done. This book is mostly about performing spatial measurements through the statistical sampling of images; it is a text on classical stereology as John Hilliard saw it. His vision of the sub...
Practical Stereology
vi on geometric probability is included, students can be expected to create a few simple programs like those shown, but for other geometries. I am indebted to Tom Hare for critical reviews of the material and an endless enthusiasm to debate and derive stereological relationships; to John Matzka at Plenum Press for patiently instructing me in the intricacies of typesetting; to Chris Russ for helping to program many of these measurement techniques; and especially to Helen Adams, both for her patience with my creative fever to write yet another book, and for pointing out that the title, which I had intended to contrast to "theoretical stereology," can also be understood as the antonym of "impra...
Unbiased Stereology
Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience. To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.
Basic Stereology for Biologists and Neuroscientists
Stereological techniques allow biologists to create quantitative, three-dimensional descriptions of biological structures from two- dimensional images of tissue viewed under the microscope. For example, they can accurately estimate the size of a particular organelle, the total length of a mass of capillaries, or the number of neurons or synapses in a particular region of the brain. This book provides a practical guide to designing and critically evaluating stereological studies of the nervous system and other tissues. It explains the basic concepts behind design-based stereology and how to get started. Also included are detailed descriptions of how to prepare tissue appropriately, perform pilot studies and decide on the appropriate sampling strategy, and account for phenomena such as tissue shrinkage. Numerous examples of applications of stereological methods that are applicable to studies of the central system and a wide variety of other tissues are explained. The book is therefore essential reading for neurobiologists and cell biologists interested in generating accurate representations of cell and tissue architecture.
Neurostereology
Stereological methods provide researchers with unparalleled quantitative data from tissue samples and allow for well-evidenced research advances in a broad range of scientific fields. Presenting a concise introduction to the methodology and application of stereological research in neuroscience, Neurostereology provides a fuller understanding of the use of these methods in research and a means for replicating successful scientific approaches. Providing sound footing for future research, Neurostereology is a useful tool for basic and clinical researchers and advanced students looking to integrate these methods into their research.
UNBIASED STEREOLOGY
In Unbiased Stereology, the authors present a practical guide to making unbiased 3D measurements using microscopy. Stereology is the statistical inference of geometric parameters from spatially sampled information; it is considered unbiased when the test probes are positioned in an appropriately random manner. Now that unbiased stereology is required for nerve-cell counts in neurotoxicology by the FDA and by many journals before the acceptance of an article or study, this book is essential reading. It includes only those stereological techniques that have been tried and tested by actual application. Specific topics covered include: random sampling and random geometry; the estimation of feature volume, surface, length, number and mean particle size; as well as statistics for stereologists.
Unbiased Stereology
This second edition expands the original guide to 3-D measurements in microscopy using stereological methods by adding a section on 2-D stereology, or perimetrics. There is also a chapter on single object stereology, which permits the measurement of total volume, surface and feature length of single arbitrary objects.
