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This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
First multi-year cumulation covers six years: 1965-70.
Summary of a workshop sponsored by OECD's Road Research Program.
The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography
It has been around since the first rear-impact automobile accident and it will continue to be a problem as long as humans have large, heavy heads perched on slender, highly mobile cervical spines. The subject is whiplash, and some of the brightest minds on the topic gathered in Banff, Alberta, Canada, for the Eighth International Symposium by the Physical Medicine Research Foundation. Editor Dr. Murray E. Allen, Chairman of the Symposium, has collected the findings in Musculoskeletal Pain Emanating From the Head and Neck: Current Concepts in Diagnosis, Management, and Cost Containment to help physicians, physical therapists, chiropractors, and researchers better understand “the new whiplas...
For this ready reference, the internationally renowned authority in the field, Roland Kontermann, has assembled a team of outstanding contributors from industry and academia to convey the worldwide knowledge on modifying therapeutic proteins in order to optimize their pharmacological potential. The result is a comprehensive work covering all approaches and aspects of the topic in one handy volume, making this indispensable reading for companies and research institutions working on the development of biopharmaceuticals.
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The second of a series of Yearbooks in the Work Life 2000 programme, preparing for the Work Life 2000 Conference in Malmö 22 - 25 January 2001, as a part of the Swedish Presidency of the European Union