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Biology of Trypanosoma cruzi
Trypanosoma cruzi, an important zoonotic protozoan that causes Chagas disease, affects at least 8 million people in Latin America. Chagas disease is an important life-long infection in humans that can be divided into distinct clinical stages: the acute phase, where patient symptoms can vary from asymptomatic to severe; the indeterminate form, which is usually asymptomatic; and the chronic phase, where cardiomyopathy and/or digestive megasyndromes appear. In addition to its medical importance, T. cruzi is an interesting biological model for studying processes such as: (1) cell differentiation, where a non-infective stage transforms into an infective one; (2) cell invasion, where the infective...
Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma Cruzi for Studies of Parasitic Biology
This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications.
Encyclopedia of Information Science and Technology
"This set of books represents a detailed compendium of authoritative, research-based entries that define the contemporary state of knowledge on technology"--Provided by publisher.
Immune Correlates of Protection for Emerging Diseases – Lessons from Ebola and COVID-19
Correlates of Protection (CoP) are biological parameters present in vaccinated or naturally infected individuals that predict levels of protection against an infectious disease. CoP facilitate vaccine licensing since they enable: a) the selection of antigen composition of a vaccine; b) the assessment of vaccine efficacy in clinical trials without exposure of participants to natural infection; and c) bridging between first- and second-generation vaccines.
Vaccination of Special Populations: Protecting the Vulnerable
Vaccines prevent 3 million of deaths every year and are a crucial to combat antimicrobial resistance. An optimal implementation of existing vaccines could help to avert up to 1.5 million deaths as well as substantial disabilities. Indeed, vulnerable populations (VPs), including pregnant women, newborns, preterm infants, elderly and patients affected by chronic diseases are frequently undervaccinated and/or at risk of reduced vaccine efficacy, presenting a major health and economic burden to society. Vaccine development programs mainly focus on healthy populations; therefore, vaccination strategies are often based on data arising from healthy subjects.
Women, Business and the Law 2021
Women, Business and the Law 2021 is the seventh in a series of annual studies measuring the laws and regulations that affect women’s economic opportunity in 190 economies. The project presents eight indicators structured around women’s interactions with the law as they move through their lives and careers: Mobility, Workplace, Pay, Marriage, Parenthood, Entrepreneurship, Assets, and Pension. This year’s report updates all indicators as of October 1, 2020 and builds evidence of the links between legal gender equality and women’s economic inclusion. By examining the economic decisions women make throughout their working lives, as well as the pace of reform over the past 50 years, Women, Business and the Law 2021 makes an important contribution to research and policy discussions about the state of women’s economic empowerment. Prepared during a global pandemic that threatens progress toward gender equality, this edition also includes important findings on government responses to COVID-19 and pilot research related to childcare and women’s access to justice.
Baculovirus and Insect Cell Expression Protocols
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envel...
Darcey Bussell - Evolved
The first book from Darcey Bussell in over six years, retired darling of the British Ballet and beloved judge of Strictly Come Dancing, this publishing extravaganza coincides with the the superstar ballerina's 50th birthday. Exquisitely produced, the book is filled with remarkable images of Dame Darcey in various notable locations, such as a pod of the London Eye, on top of the Victoria and Albert memorial, and performing at worldwide events, like the Olympics opening ceremony. The collection includes rare and unseen moments of Darcey shot by some of the most famous photographers, including Lord Snowden, Mario Testing and Annie Liebovitz, in locations beyond the stage - including rehearsals, fashion shoots and more - which are accompanied by behind-the-scenes stories and personal anecdotes. A fitting testament to one of our true national treasures, this glorious and charming book is a wonder to enjoy for years to come.
V Jornada Integrada do UNIFSM
V JORNADA INTEGRADA DO UNIFSM
