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Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma Cruzi for Studies of Parasitic Biology
This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications.
Biology of Trypanosoma cruzi
Trypanosoma cruzi, an important zoonotic protozoan that causes Chagas disease, affects at least 8 million people in Latin America. Chagas disease is an important life-long infection in humans that can be divided into distinct clinical stages: the acute phase, where patient symptoms can vary from asymptomatic to severe; the indeterminate form, which is usually asymptomatic; and the chronic phase, where cardiomyopathy and/or digestive megasyndromes appear. In addition to its medical importance, T. cruzi is an interesting biological model for studying processes such as: (1) cell differentiation, where a non-infective stage transforms into an infective one; (2) cell invasion, where the infective...
Leitura, acessibilidade e atuação d@s Bibliotecári@s
Este livro é uma iniciativa do Biblio Fora da Caixa, um canal de comunicação que divulga pessoas, instituições e projetos que são "fora da caixa", diferenciais no que fazem. Durante o período de pandemia do Coronavírus (COVID-19) convidamos diversos profissionais, docentes e pesquisadores em geral para apresentarem suas pesquisas e experiências, com temas diversos. Estas discussões resultaram em textos inéditos, que agora são publicados em forma de ebook. Para esta edição, selecionamos os textos de convidados do canal que escreveram capítulos sobre leitura e experiências no clube do livro Maranhão, a atuação do bibliotecário escolar em rede, sobre bibliotecas universitárias de campi avançados, informática para pessoas com deficiência intelectual e informações acessíveis para usuários surdos em repositórios digitais, sob a perspectiva do bibliotecário. Esta edição, compõe o primeiro volume da coleção intitulada 'Pesquisas e experiências compartilhadas no Biblio Fora da Caixa'. Neste sentido, este ebook explora as perspectivas de atuação e fomenta discussões na área da Biblioteconomia.
Festival and Special Event Management
Festivals and special events have grown into a massive industry worldwide, generating billions of dollars for regional centres, states and countries. Festivals and special events bring people together to celebrate, to remember, to support and to identify as a community or nation. Festival and Special Event Management 2nd edition provides a comprehensive overview of the theory and procedures associated with the management of festivals and special events. The new edition features new developments, professional ?tools? and a discussion of the role of technology.
Occupational Safety and Hygiene
Occupational Safety and Hygiene presents selected papers from the International Symposium on Occupational Safety and Hygiene – SHO2013 (Guimarães, Portugal, 14-15 February 2013), which was organized by the Portuguese Society for Occupational Safety and Hygiene (SPOSHO). The contributions from 15 different countries focus on: - Occupational safety - Risk assessment - Safety management - Ergonomics - Management systems - Environmental ergonomics - Physical environments - Construction safety - Human factors The papers included in the book are mainly based on research carried out at universities and other research institutions, but they are also based on practical studies developed by Occupational Health & Safety (OHS) practitioners within their companies. As a result, this book will be useful to get acquainted with the state-of-the-art of the research within the aforementioned domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide.
Festival and Event Tourism
Festivals and events vary from small, neighbourhood celebrations through to mega gatherings, and both can be attractive to tourists. They come with their own unique challenges and opportunities however, which means destinations must carefully consider their responsibility to local people, and host them in a sustainable manner. Covering important issues such as the marketing, branding and promotion of events, this book also unravels the opportunities and challenges associated with sustainable festivals and events. It uses an array of case studies and a global author team to provide an important resource for tourism and event researchers and professionals.
Organophosphorus Chemistry
Filling the gap for an up-to-date reference that presents the field of organophosphorus chemistry in a comprehensive and clearly structured way, this one-stop source covers the chemistry, properties, and applications from life science and medicine. Divided into two parts, the first presents the chemistry of various phosphorus-containing compounds and their synthesis, including ylides, acids, and heterocycles. The second part then goes on to look at applications in life science and bioorganic chemistry. Last but not least, such important practical aspects as 31P-NMR and protecting strategies for these compounds are presented. For organic, bioinorganic, and medicinal chemists, as well as those working on organometallics, and for materials scientists. The book, a contributed work, features a team of renowned scientists from around the world whose expertise spans the many aspects of modern organophosphorus chemistry.
O Lobo Branco
Dois jovens normais ou dois heróis? Essa história conta a aventura de dois amigos, Blanko Sloan e Conner Monroe, garotos que são surpreendidos com uma parte do mundo que não conheciam, onde monstros a todo momento estarão os perseguindo devido suas auras extremamente atrativas herdadas pelos elementos da tempestade e do fogo. A dúvida de toda essa história é: A profecia é correta e precisa? O destino de Blanko e Conner realmente está selado? Quem vence, tempestade ou fogo?
