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Digital Droplet PCR on Disk
Abstract: Existing systems for digital droplet PCR (ddPCR) either suffer from low integration or are difficult to introduce to mass fabrication. Here we present an integrated system that is compatible to mass fabrication and combines emulsification, PCR, and fluorescence readout in a single chamber within a disposable cartridge (disk). Droplets are generated by injecting the sample into fluorinated oil via centrifugal step emulsification. The resulting emulsion is aligned in the PCR and readout zone by capillary action. During thermocycling, gas bubbles generated by degassing are removed by capillary driven transport through tapered regions in the PCR chamber. Thereby, the positioning of the...
Ecology and Management of Giant Hogweed (Heracleum Mantegazziannum)
This book is a compendium of the current knowledge on the invasive plant, giant hogweed (Heracleum mantegazzianum) that spread from Caucasus across Europe, creating serious problems displacing and overtaking native plants. Topics covered include: taxonomy, genetics, reproductive ecology, and invasion dynamics at regional and local scales. Intended for students, researchers and practitioners in agriculture, conservation, weed science and plant ecology, this book also reviews the possibilities of mechanical, chemical and biological control, and control by grazing.
Critical Care Nursing of Older Adults
2010 AJN Book of the Year Award Winner in Critical Care--Emergency Nursing! Designated a Doody's Core Title! "This evidence-based book is an excellent reference for ensuring high-quality management of the elderly and of their particular needs in the critical care setting." --AJN "[This] book's contents run the gamut of elder problems and care: physiology, pharmacology, nutrition, restraints, substance abuse....it is a compendium that can be used as a text or a resource." --Claire M. Fagin, PhD, RN, FAAN (From the Foreword) This book is an evidence-based, best-practices guide that directs the bedside care of critically ill elders. The book serves as a reference on major clinical issues for nu...
AIDS: Virus- or Drug Induced?
Despite enormous efforts, over 100,000 papers and over $22 billion spent by the US taxpayer alone, the HIV-AIDS hypothesis has failed to produce any public health benefits, no vaccine, no effective drug, no prevention, no cure, not a single life saved. Is the science system to be blamed? Has science failed to reveal the truth about AIDS? In AIDS: Virus or Drug Induced?, two dozen scientists, scholars and journalists have investigated the status quo of AIDS research. Most of them have questioned the HIV-AIDS hypothesis before, but have since been censored, and sociologically excluded from AIDS research, politics and journalism. Here they are united for the first time to put on trial the HIV-A...
Diagnostic Nuclear Medicine
Designed to present students and professionals with a comprehensive update of recent developments not found in other textbooks on the subject, the various clinical applications of nuclear medicine techniques are considered here, and due attention is also given to radiopharmaceuticals, equipment and instrumentation, reconstruction techniques and the principles of gene imaging
Practical Guide to Environmental Management
Aimed at environmental and business professionals, this text explains how to create and maintain an effective corporate environmental management program. Corporate environmental lawyer Friedman begins with an overview of the history of environmental law. Other topics include, for example, reducing w
Replacement of Renal Function by Dialysis
The leading Textbook on the subject. A completely rewritten and up-to-date fifth edition, based upon the highly respected fourth edition, edited by C. Jacobs, C.M. Kjellstrand, K.M. Koch and J.F. Winchester. This new edition is truly global in scope and features the contributions of the top experts from around the world.
Epidemiology and Medical Statistics
This volume, representing a compilation of authoritative reviews on a multitude of uses of statistics in epidemiology and medical statistics written by internationally renowned experts, is addressed to statisticians working in biomedical and epidemiological fields who use statistical and quantitative methods in their work. While the use of statistics in these fields has a long and rich history, explosive growth of science in general and clinical and epidemiological sciences in particular have gone through a see of change, spawning the development of new methods and innovative adaptations of standard methods. Since the literature is highly scattered, the Editors have undertaken this humble ex...
Cardiovascular Plaque Rupture
Illustrating the development and determinants of possible plaque rupture, Cardiovascular Plaque Rupture provides an in-depth review of the pathology, etiology, cellular and molecular biology, diagnosis, and treatment of plaque in the cardiovascular system-exploring the roles of physical stress, inflammation, and infectious agents on the growth of c
