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Low-Cost Methods for Molecular Characterization of Mutant Plants
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Fungi and Food Spoilage
This book is designed as a laboratory guide for the food microbiologist, to assist in the isolation and identification of common food-borne fungi. We emphasise the fungi which cause food spoilage, but also devote space to the fungi commonly encountered in foods at harvest, and in the food factory. As far as possible, we have kept the text simple, although the need for clarity in the descriptions has necessitated the use of some specialised mycological terms. The identification keys have been designed for use by microbiologists with little or no prior knowledge of mycology. For identification to genus level, they are based primarily on the cultural and physiological characteristics of fungi g...
DNA Fingerprinting in Plants
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p
Components of Variance
Identifying the sources and measuring the impact of haphazard variations are important in any number of research applications, from clinical trials and genetics to industrial design and psychometric testing. Only in very simple situations can such variations be represented effectively by independent, identically distributed random variables or by random sampling from a hypothetical infinite population. Components of Variance illuminates the complexities of the subject, setting forth its principles with focus on both the development of models for detailed analyses and the statistical techniques themselves. The authors first consider balanced and unbalanced situations, then move to the treatme...
Biotechnologies for Plant Mutation Breeding
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
The Domestic Animal/wildlife Interface
This volume addresses the problems of disease in both wildlife and domestic animals and the transmission of disease between the two populations. It examines ways in which we can conserve and manage animals in both sectors for optimal health and production, while preserving ecological communities.
Mechanization of Horticulture
''The Book entitled " Mechanization of Horticulture" deals with different operations for production and post-harvest processing and management of horticultural crops and commodities. This book is essentially a Monograph which has been divided into 16 chapters. Chapters I and II summaries the current horticultural scenario as well as status of agricultural mechanization in india. Chapter III through XII deal with various operations, viz. seed bed preparation, planting, nursery raising, soil working tools transplanting of seedlings weeding and mulching, pruning, spraying and harvesting. Information regarding different types of equipment available in India and abroad has been included in these chapters. The last three chapters viz. chapters XIII through XVI deal with packaging of horticultural produce post harvest management and storage, green house design and construction as well as irrigation systems. Thus, it will serve as a useful reference book for teachers, researchers, extension workers, farmers, students as well as other state holder.''
Food Security in Africa
'As they often do, Jamie Morrison and Alexander Sarris have provided researchers, policy-makers, and the interested public with the firm empirical grounding needed for sound agricultural development policies. They have synthesized from a rich and varied set of country studies a unique contribution to one of the key challenges of our times increasing the productivity of smallholder food production in the age of globalization.' Timothy A. Wise, Tufts University, US 'Food security has been a major concern in Africa for decades, and a more pressing problem with recent increases in food prices. The editors and contributors to this volume are experts in the field and should be commended for a time...
Citrus Oils
World production of citrus fruits is still growing. At present, about 30 percent of that yield is devoted to industrial production, mostly on those essential oils and juices used in foods, pharmaceuticals, and cosmetics. Covering research reported in the literature over the past ten years, this book presents the most current research available
