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Mycobacterium Tuberculosis Protocols
The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been ...
Mycobacteria Protocols
Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.
The Dracula Secrets
Since the first publication of Dracula in 1897, there have been suggestions that the book's protagonist is more closely associated with Jack the Ripper than a Transylvanian count. In The Dracula Secrets, historian Neil R. Storey undertakes an in-depth investigation of the sources used by Stoker during the writing of his seminal masterpiece. Painting an evocative portrait of Stoker, his influences, his friends and the London he frequented in the late nineteenth century, Storey explores how Stoker created Dracula out of the climate of fear that was created by the Whitechapel murders in 1888. Indeed he asks, did Stoker know Jack the Ripper personally and hide the clues to this terrible knowledge in his book? Having gained unprecedented access to the unique archive of one of Stoker's most respected friends and the dedicatee of Dracula, Storey sheds new light on both Stoker and Dracula, and reveals startling new links between Stoker's creation and the most infamous serial killer of all time.
A LITTLE TRIGGER
A free digital sampler containing short story 'The Thing About Cassandra' and a preview of Neil Gaiman's new collection, TRIGGER WARNING. Neil Gaiman on 'The Thing About Cassandra' 'When I was about fourteen, it seemed much easier to imagine a girlfriend than to have one - that would involve actually talking to a girl, after all. So I would, I decided, write a girl's name on the cover of my exercise books and deny all knowledge of her when asked, thus, I fondly imagined, causing everyone to think that I actually had a girlfriend. I do not believe it worked. I never actually got around to imagining anything about her but the name.' A LITTLE TRIGGER also includes a preview of TRIGGER WARNING, the new short story collection from Neil Gaiman.
Bram Stoker: Author of Dracula
Previously unpublished research sheds new light on how Bram Stoker researched and wrote Dracula and the people who inspired his characters. Bram Stoker: Author of Dracula is an affectionate and revealing biography of the man who created the vampire novel that would define the genre and lead to a new age in Gothic horror literature. Based on decades of painstaking research in libraries, museums, and university archives and privileged access to private collections on both sides of the Atlantic, the private letters of Bram and the reminiscences of those who knew him not only shed new light on Stoker's ancestry, his life, loves and friendships they also reveal more about the places and people wh...
Dracula
Before acclaimed playwright and filmmaker Neil LaBute became the creator and showrunner of Syfy’s hit series Van Helsing, he had already adapted Dracula for the stage—with a fierce female Van Helsing as the vampire hunter. In this masterful adaptation, Neil LaBute brings a rich theatricality and his provocative way with language and story to the world of Count Dracula, Van Helsing, Jonathan Harker, and his beloved Mina—this time, with very much a mind of her own—infusing the classic gothic tale of terror, obsession, and pathos with a modern edge. Chilling yet stylish in its atmosphere, dark yet deeply human in its emotional impact, Neil LaBute’s Dracula: A Thriller in 2 Acts is a tribute to both LaBute’s dramatic vision and the timelessness of Stoker’s novel.
Angiogenesis Protocols
In the last few years, we have been deluged with information on ang- genesis. Scientists and the public at large are exposed daily to this “new” science, not just in specialist journals and texts, but in the tabloid press, where popular articles refer to angiogenic therapies as magic bullets and miracle cures for cancer, arthritis, retinopathies, heart disease, and circulatory problems. Is there no ill this approach will not cure? The fact that so much time, effort, and resource have been and continue to be dedicated to this new science is clear testament to its importance. Yet many fundamental aspects of angiogenesis remain poorly und- stood, in particular cues that activate the process...
Molecular Analysis of Cancer
Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chap...
Molecular Pathology Protocols
The era of molecular pathology has arrived. From its promising beg- nings in research laboratories, the field has grown, and continues to grow, to become a vital part of the care of an ever-increasing number of patients. Because of its recent emergence from the research taboratory, many molecuIar pathology protocols we still to be found in the primary litcramre, and have not appeared in a text. MO~PCU~Q~ Padhoiogy Protocob contains la- ratory protocoIs that have been developed by many of the authors for use in clinical molecular pathology laboratories and describe in detail Row to perform these assays. This book is therefore intended for clinical laboratory use by medical technologists and p...
Vision Research Protocols
Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work in vision research today.
