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Characterization of a Setup to Test the Impact of High-amplitude Pressure Waves on Living Cells
Abstract: The impact of pressure waves on cells may provide several possible applications in biology and medicine including the direct killing of tumors, drug delivery or gene transfection. In this study we characterize the physical properties of mechanical pressure waves generated by a nanosecond laser pulse in a setup with well-defined cell culture conditions. To systematically characterize the system on the relevant length and time scales (micrometers and nanoseconds) we use photon Doppler velocimetry (PDV) and obtain velocity profiles of the cell culture vessel at the passage of the pressure wave. These profiles serve as input for numerical pressure wave simulations that help to further quantify the pressure conditions on the cellular length scale. On the biological level we demonstrate killing of glioblastoma cells and quantify experimentally the pressure threshold for cell destruction
Sobrevivência, Integração e Diferenciação Neural de Células-Tronco Mesenquimais no Sistema Nervoso
É crescente o interesse por terapia celular restauradora. No particular caso do Sistema Nervoso Central, células-tronco nos apresentam o potencial de reverter sequelas neurológicas graves ou mesmo tratar doenças incuráveis como doença de Parkinson, Acidente Vascular Encefálico, Traumatismo craniano e medular, entre outras. Diversos tipos celulares vem sendo investigados para uso em transplante neural. Cada um deles apresenta vantagens e desvantagens. Enquanto alguns tipos celulares (como células-tronco neurais) são de difícil isolamento, outros (como células embrionárias) trazem o perigo de degeneração cancerosa. Células-tronco mesenquimais, por outro lado, são de fácil isol...
Advances in Minimally Invasive Surgery and Therapy for Spine and Nerves
Radiologists, orthopedic and neurological surgeons present the different minimally invasive methods. Peripheral nerve problems and problems concerning differential diagnosis in special situations such as between radicular and peripheral nerve trunk lesions are discussed, pinpointing the significance of different diagnostic tools. Minimally invasive techniques, utilized nowadays to minimize bone demolition, scarring and risk of recurrence are analyzed. Microdiscectomy is compared with the results of intradiscal techniques, and new methods are discussed facing problems such as epidural fibrotisation, microinstability, osteoporotic or neoplastic or posttraumatic vertebral lesions.
Thymic Tumors
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Basic Clinical Neuroscience
Basic Clinical Neuroscience offers medical and other health professions students a clinically oriented description of human neuroanatomy and neurophysiology. This text provides the anatomic and pathophysiologic basis for understanding neurologic abnormalities through concise descriptions of functional systems with an emphasis on medically important structures and clinically important pathways. It emphasizes the localization of specific anatomic structures and pathways with neurological deficits, using anatomy enhancing 3-D illustrations. Basic Clinical Neuroscience also includes boxed clinical information throughout the text, a key term glossary section, and review questions at the end of each chapter, making this book comprehensive enough to be an excellent Board Exam preparation resource in addition to a great professional training textbook. The fully searchable text will be available online at thePoint.
Deep Brain Stimulation in Neurological and Psychiatric Disorders
This important book discusses today’s most current and cutting-edge applications of Deep Brain Stimulation (DBS). The book begins with reviews of the functional anatomy and physiology of motor and nonmotor aspects of the basal ganglia and their connections which underlie the application of DBS to neurological and psychiatric disorders. This is followed by proposed mechanisms of action of DBS based on functional neuroimaging and neurophysiologic studies in animals and man.
Wound Regeneration and Repair
In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls. Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.
Molecular Dynamics
Molecular Dynamics is a two-volume compendium of the ever-growing applications of molecular dynamics simulations to solve a wider range of scientific and engineering challenges. The contents illustrate the rapid progress on molecular dynamics simulations in many fields of science and technology, such as nanotechnology, energy research, and biology, due to the advances of new dynamics theories and the extraordinary power of today's computers. This second book begins with an introduction of molecular dynamics simulations to macromolecules and then illustrates the computer experiments using molecular dynamics simulations in the studies of synthetic and biological macromolecules, plasmas, and nanomachines. Coverage of this book includes: Complex formation and dynamics of polymers Dynamics of lipid bilayers, peptides, DNA, RNA, and proteins Complex liquids and plasmas Dynamics of molecules on surfaces Nanofluidics and nanomachines
