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The Gb3-enriched CD59/flotillin Plasma Membrane Domain Regulates Host Cell Invasion by Pseudomonas Aeruginosa
Abstract: The opportunistic pathogen Pseudomonas aeruginosa has gained precedence over the years due to its ability to develop resistance to existing antibiotics, thereby necessitating alternative strategies to understand and combat the bacterium. Our previous work identified the interaction between the bacterial lectin LecA and its host cell glycosphingolipid receptor globotriaosylceramide (Gb3) as a crucial step for the engulfment of P. aeruginosa via the lipid zipper mechanism. In this study, we define the LecA-associated host cell membrane domain by pull-down and mass spectrometry analysis. We unraveled a predilection of LecA for binding to saturated, long fatty acyl chain-containing Gb3...
Quantification of Nanoscale Forces in Lectin-mediated Bacterial Attachment and Uptake Into Giant Liposomes
Abstract: Interactions of the bacterial lectin LecA with the host cells glycosphingolipid Gb3 have been shown to be crucial for the cellular uptake of the bacterium Pseudomonas aeruginosa. LecA-induced Gb3 clustering, referred to as lipid zipper mechanism, leads to full membrane engulfment of the bacterium. Here, we aim for a nanoscale force characterization of this mechanism using two complementary force probing techniques, atomic force microscopy (AFM) and optical tweezers (OT). The LecA-Gb3 interactions are reconstituted using giant unilamellar vesicles (GUVs), a well-controlled minimal system mimicking the plasma membrane and nanoscale forces between either bacteria (PAO1 wild-type and L...
The Badger
UW Archives holds up to three copies of each volume of the yearbook from its initial publication in 1884 to its final publication in 2014 (129 volumes). The publication of the yearbook did not become annual until 1887, as such there are no yearbooks for 1885 or 1886. The only other interruption in yearbooks was for the years 1973 and 1974. There are still yearbooks from these years, but they were published by the Wisconsin Alumni Association rather than the student body, as such they are spare, consisting mostly of portraits of students. UW Archives currently holds at least one copy of every published volume. The 1st copy of each volume is held onsite at UW Archives while the second and third copies, where they exist, are held offsite.
Annette
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Annette and Sylvie
The book, "" Annette and Sylvie: Being Volume One of The Soul Enchanted Part - I "", has been considered important throughout the human history, and so that this work is never forgotten we have made efforts in its preservation by republishing this book in a modern format for present and future generations. This whole book has been reformatted, retyped and designed. These books are not made of scanned copies and hence the text is clear and readable.
