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Isolation and Molecular Characterization of Circulating Tumor Cells
Numerous studies have shown that elevated levels of circulating tumor cells (CTCs) in blood of cancer patients are associated with poor response to treatment and inferior survival probabilities. Despite this clinical significance, the molecular biology of CTCs remains poorly understood. The paucity in molecular information can be attributed to the tremendous technical challenges involved in isolating these extremely rare cells. Recent technological advancements in rare-cell technology, however, have allowed for the reliable enrichment and isolation of CTCs. Consequently, the use of recently developed molecular approaches —e.g., multiplexed QPCR, microarray, and next generation sequencing analyses— to profile CTCs have provided novel insights into the molecular makeup of these tumor cells. This book discusses approaches for enrichment and isolation of CTCs as well as recent advances in comprehensive molecular profiling of CTCs using cutting-edge omics technology.
Circulating Tumor Cells
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Circula ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.
The Book of Ho'oponopono
A simple practice to heal your past and cleanse negative memories to live a more peaceful and harmonious life • Details how to apply Ho'oponopono to deal with traumatic past events, destructive thought patterns, family dynamics, daily annoyances, or any other disagreeable event in your life, from traffic jams to relationship break-ups • Draws on the new science of epigenetics and quantum physics to explain how Ho'oponopono works • Explains how the trauma of past events can cloud your perceptions and reveals how to break free from the weight of your memories Based on an ancestral Hawaiian shamanic ritual, the healing practice of Ho'oponopono teaches you to cleanse your consciousness of ...
Nutritional Genomics
The definitive guide to the basic principles and latest advances in Nutritional Genomics Though still in its infancy, nutritional genomics, or "nutrigenomics," has revealed much about the complex interactions between diet and genes. But it is in its potential applications that nutrigenomics promises to revolutionize the ways we manage human health and combat disease in the years ahead. Great progress already has been made in modeling "personalized" nutrition for optimal health and longevity as well as in genotype-based dietary interventions for the prevention, mitigation, or possible cure of a variety of chronic diseases and some types of cancer. Topics covered include: * Nutrients and gene ...
The Decision Tree
For all the talk about personalized medicine, our health care system remains a top-down, doctor-driven system where individuals are too often bit players in their own health decisions. In The Decision Tree, Thomas Goetz proposes a new strategy for thinking about health, one that applies cutting-edge technology to put us at the center of the equation and explains how the new frontier of health care can impact each of our lives.
Advances in Medical Biochemistry, Genomics, Physiology, and Pathology
The pace and sophistication of advances in medicine in the past two decades have necessitated a growing need for a comprehensive reference that highlights current issues in medicine. Each volume in the Current Issues in Medicine series is a stand‐alone text that provides a broad survey of various critical topics—all accomplished in a user-friendly yet interconnected format. The series not only highlights current advances but also explores related topics such as translational medicine, regulatory science, neglected diseases, global pandemics, patent law, immunotoxicology, theranostics, big data, artificial intelligence, novel imaging tools, combination drug products, and novel therapies. ...
Cancer Genetics and Genomics for Personalized Medicine
This book covers almost all fields of cancer genetics and genomics for personalized medicine. Targeted therapy, or precision medicine, or personalized medicine is becoming a standard treatment for many diseases, including cancer. However, how much do we know about the personalized medicine approach? This lucid book helps undergraduate and graduate students, professional researchers, and clinicians to better understand the key concept of personalized medicine. The most up-to-date topics on personalized medicine in this book cover the recent trends in and updates on lung, gastric, liver, breast, and other types of cancers. Circulating tumor cell, cell-free circulating DNA, and microRNAs are di...
The Role of Exercise in Anti-Aging
This is a book written by a Medical Doctor who specializes in the preventive aspect of Sports Medicine, and is all about his experience in looking after patients, keeping abreast with the latest advances, and competing in various sports,. It discusses the popular modes of exercise so that the reader need not have to try them out himself, and explores the newer medical concepts. It is written from practical experience , from treating paients, observing those who age around us, and from applying these very principles on the author himself. The proof is in the pudding, and many examples of the healthy elderly are shown.
