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Handbook of Fluorescence Spectroscopy and Imaging
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
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Bio-nanoimaging
Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers - Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme...
Smart Micro- and Nanomaterials for Pharmaceutical Applications
Smart drug delivery refers to a targeted drug delivery or precision drug delivery system that allows drugs to be administered to a specific location in the body or at a specific time with enhanced precision and control. This approach has several advantages, including maximizing the therapeutic effects of a drug while minimizing side effects. This book presents various stimuli-responsive micro- and nanomaterials for pharmaceutical industries. This volume: Covers the global market perspective of micro- and nano-smart materials in pharmaceutical industries. Details various processing routes. Discusses mechanisms for target release. Addresses applications in oral drug delivery, anticancer agents, anti-tumor drug delivery, and drugs for management of infection. This reference work is written to support researchers in the fields of materials engineering and biotechnology with the goal of improving the diagnosis and treatment of disease and patient quality of life.
Handbook of Biophotonics
This new handbook covers the world of biophotonics not only geographically -- with the editors coming from different continents -- but also in terms of content, since the authors come from the whole spectrum of biophotonic basic and applied research. Designed to set the standard for the scientific community, these three volumes break new ground by providing readers with the physics basics as well as the biological and medical background, together with detailed reports on recent technical advances. The Handbook also adopts an application-related approach, starting with the application and then citing the various tools to solve the scientific task, making it of particular value to medical doctors. Divided into several sections, the first part offers introductory chapters on the different fields of research, with subsequent parts focusing on the applications and techniques in various fields of industry and research. The result is a handy source for scientists seeking the basics in a condensed form, and equally a reference for quickly gathering the knowledge from neighboring disciplines. Absolutely invaluable for biophotonic scientists in their daily work.
Perfusion Cell Culture Processes for Biopharmaceuticals
This book is a monography about perfusion cell cultures for the production of biopharmaceuticals, such as therapeutic proteins (i.e. biomolecules like monoclonal antibodies), and describes the fundamentals, design and operation of these processes. Context is given in the first chapters to understand the state-of-the-art of the technology. We then give an overview of the challenges and objectives in operating mammalian cell perfusion cultures and provide guidelines for the design and setup of lab-scale bioreactor systems, and the required control structure to achieve stable operation. Scale-down devices and PAT tools are described in the context of continuous manufacturing and guidelines for process optimization are given using a variety of case studies to illustrate different approaches. Scale-up is also adressed with a strong focus on bioreactor aeration and mixing, shear stress and cell retention device. Finally, a general introduction for the application of mechanistic and statistic models in bioreactor process development and optimization is given in the last chapter.
Analytical Techniques In DNA Sequencing
While one marvels at the DNA generated by the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding. Analytical Techniques in DNA Sequencing takes a look at the various DNA
