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Single-Domain Antibodies: Biology, Engineering and Emerging Applications
Single-domain antibodies (sdAbs) represent the minimal antigen binding-competent form of the immunoglobulin domain and have unique properties and applications. SdAbs are naturally produced as the variable domains of the heavy chain-only antibodies of camelid ruminants and cartilaginous fishes, but can also be engineered synthetically from autonomous human or mouse VH or VL domains. The scope of this research topic and associated e-book covers current understanding and new developments in (i) the biology, immunology and immunogenetics of sdAbs in camelids and cartilaginous fishes, (ii) strategies for sdAb discovery, (iii) protein engineering approaches to increase the solubility, stability and antigen-binding affinity of sdAbs and (iv) specialized applications of sdAbs in areas such diagnostics, imaging and therapeutics.
Science with the Atacama Large Millimeter Array:
Currently under construction in Northern Chile, the Atacama Large Millimeter Array (ALMA) is the most ambitious astronomy facility under construction. This book describes the enormous capabilities of ALMA, the state of the project, and most notably the scientific prospects of such a unique facility. The book includes reviews and recent results on most hot topics of modern astronomy. It looks forward to the revolutionary results that are likely to be obtained with ALMA.
Super-Resolution Imaging in Biomedicine
This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.
Universities and Academic Labour in Times of Digitalisation and Precarisation
This book provides a critical perspective on the digitalisation of universities and precarisation of academic labour. While research and teaching become more virtual and digital at universities, academic labour is becoming more and more casualised and temporary. This book aims to analyse and theorise academic labour and study the experiences academic workers have made at universities that are shaped by economic, political and cultural contexts. It will be a valuable tool for international scholars and students of subjects such as media, communication and cultural studies, sociology, education, management and labour studies. The insights will also be of particular relevance for unions and other initiatives that are concerned about the working conditions at universities.
