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Analyzing T Cell Responses
Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials (1-3). To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined, since it would facilitate the rapid comparison of these various formulations. Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated...
Accurate Quantification of Chromosomal Lesions Via Short Tandem Repeat Analysis Using Minimal Amounts of DNA
Abstract: Background Cytogenetic aberrations such as deletion of chromosome 5q (del(5q)) represent key elements in routine clinical diagnostics of haematological malignancies. Currently established methods such as metaphase cytogenetics, FISH or array-based approaches have limitations due to their dependency on viable cells, high costs or semi-quantitative nature. Importantly, they cannot be used on low abundance DNA. We therefore aimed to establish a robust and quantitative technique that overcomes these shortcomings. Methods For precise determination of del(5q) cell fractions, we developed an inexpensive multiplex-PCR assay requiring only nanograms of DNA that simultaneously measures allel...
Cancer Research
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The Mix
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MixPlus Central
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The Valve World
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Klinikleitfaden Hämatologie, Onkologie
Der Leitfaden gibt Ihnen Sicherheit im Umgang mit den Besonderheiten der Hämatologie und Onkologie. Konkret und praxisnah vermittelt er Ihnen Wissen über alle wichtigen Krankheitsbilder inkl. deren Diagnostik und Therapie. Darüber hinaus werden Sie mit verschiedenen Supportivtherapien vertraut gemacht und erhalten praktische Handlungsanleitungen im Umgang mit Notfällen in der Hämatologie/Onkologie. Die neue 2. Auflage wurde anhand neuer Leitlinien, Publikationen und Kongresspräsentationen aktualisiert. Insbesondere die Immuntherapie und die zielgerichtete Therapie wird ausführlich behandelt. Der Leitfaden eignet sich für Weiterbildungsasssitenten Hämatologie Onkologie, Innere Medizin, Allgemeinmedizin Fachärzte Innere Medizin
